Considerable economic loss occurs due to helminth parasites in country fowl throughout the world are. Though less emphasis has been placed on utilising DNA elements to study cestodes of veterinary importance, correct diagnosis is, nevertheless, critical to proper treatment and control. These are able to induce structural, biochemical and immunological changes in the host like inappetence, diarrhoea, dull hair coat and ostertagiosis. At present, control is almost exclusively based on anthelmintics. Hence, Genomic DNA analysis plays a vital role in characterizing and classifying cestode parasites.The present study revealed restriction digestion analysis of genomic DNA of Raillietina echinobothrida reference isolates collected from country chicken "Gallus domesticus" from Zambazaar market, Triplicane, Chennai (Tamil Nadu) using 9 restriction endonucleases enzymes namely HindΙΙΙ, EcoRΙ, BamHΙ, BglΙ, HaeΙΙΙ, XhoΙ, SfiΙ, NheΙ and HinfΙ revealed some differences in the restriction profile of DNA in the chick isolates. Lambda HindIII marker was used to compare the genomic DNA bands of R. echinobothrida formed in the gel. R. echinobothrida digested with the enzymes like EcoRΙ, BamHΙ, BglΙ, HaeΙΙΙ and XhoΙ compared with 23kb of lambda HindIII marker and most of them showed streaky and smeared formation of DNA and SfiΙ, NheΙ and HinfΙ showed no bands.
Curcumin, a strong anti-cancer ingredient that is found in turmeric and curry, is a prominent yellow pigment and spice. Curcumin's
anti-tumour properties include inhibition of tumour proliferation, angiogenesis, invasion, and metastasis, induction of tumour
apoptosis, increased chemotherapy sensitivity, and cell cycle and cancer stem cell regulation, suggesting that curcumin has a strong
therapeutic potential of modulating cancer progression. Curcumin's anticancer impact has been demonstrated in human breast
cancer cell lines and human prostate cancer cell lines using the Proliferating Cell Nuclear Antigen (PCNA) assay and colony formation
test, respectively. Curcumin was employed at various concentrations (1, 5, 10, 25, 50, and 100 g/ml). Curcumin inhibits cancer cell
growth in the PCNA assay, and colonies were stained with crystal violet and the number of clones in a particular region was counted
for each condition in the colony forming study. Higher concentrations of curcumin were found to be effective against cancer cell lines in this investigation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.