a b s t r a c t a r t i c l e i n f oA sediment core from Lake Butrint in southwestern Albania contains an annually-layered sequence covering the last ∼ 300 years. It provides thus an exceptionally well-dated time series to study past climate-driven environmental changes, as well as anthropogenic perturbations along the coast of the Ionian Sea. The varves are composed of organic-rich carbonate couplets and detritus-dominated clay layers. The first are deposited during spring-to-fall, and reflect the chemistry of the lake, which, in turn, is sensitive to 1) the relative importance of marine versus freshwater inputs, 2) relative evaporation rates, and 3) the productivity cycle within the lake. The detrital laminae are deposited during winter, reflecting precipitation and runoff conditions during the wet season. A 2-3‰ stable carbon isotope ratio shift in both bulk organics and authigenic carbonates was attributed to increasing eutrophication towards the end of the 20th century, and validated by historical and instrumental data. An increase in the δ 18 O of authigenic carbonates by more than 8‰ indicates the progressive salinization of the lake, which can primarily be attributed to man-made perturbations that reduced the freshwater input to the lake and/or enhanced the exchange with seawater from the nearby Ionian Sea. A recent increase in the relative evaporation versus precipitation rates may have additionally contributed to the observed 18 O enrichment in the Lake Butrint carbonates. The interdecadal cyclicity in the thickness of the detrital laminae seems to be at least partially controlled by NAO and/or ENSO-like phenomena that modulate precipitation patterns in the eastern Mediterranean. Thus, this study demonstrates the potential of combining microstratigraphic and stable isotopic tools to disentangle anthropogenic and natural environmental changes in Lake Butrint, validated by historical records.