ABSTRACT. Sequence-characterized amplified region (SCAR) markers were further developed from high-GC primer RAMP-PCRamplified fragments from Lonicera japonica DNA by molecular cloning. The four DNA fragments from three high-GC primers were successfully cloned into a pGM-T vector. The positive clones were sequenced; their names, sizes, and GenBank numbers were JYHGC1-1, 345 bp, KJ620024; YJHGC2-1, 388 bp, KJ620025; JYHGC7-2, 1036 bp, KJ620026; and JYHGC6-2, 715 bp, KJ620027, respectively. Four novel SCAR markers were developed by designing specific primers, optimizing conditions, and PCR validation. The developed SCAR markers were used for the genetic authentication
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