Two experiments were conducted to evaluate the effects of hybrid, maturity, and mechanical processing of whole plant corn on chemical and physical characteristics, particle size, pack density, and dry matter recovery. In the first experiment, hybrid 3845 whole plant corn was harvested at hard dough, one-third milkline, and two-thirds milkline with a theoretical length-of-cut of 6.4 mm. In the second experiment, hybrids 3845 and Quanta were harvested at one-third milkline, two-thirds milkline, and blackline stages of maturity with a theoretical length-of-cut of 12.7 mm. At each stage of maturity, corn was harvested with and without mechanical processing by using a John Deere 5830 harvester with an onboard kernel processor. The percentage of intact corn kernels present in unprocessed corn silage explained 62% of variation in total tract starch digestibility. As the amount of intact kernels increased, total tract starch digestibility decreased. Post-ensiled vitreousness of corn kernels within the corn silage explained 31 and 48% of the variation of total tract starch digestibility for processed and unprocessed treatments, respectively. For a given amount of vitreous starch in corn kernels, total tract starch digestibility was lower for cows fed unprocessed corn silage compared with processed corn silage. This suggests that processing corn silage disrupts the dense protein matrix within the corn kernel where starch is embedded, therefore making the starch more available for digestion. Particle size of corn silage and orts that contained corn silage was reduced when it was processed. Wet pack density was greater for processed compared with unprocessed corn silage.
Screening accessions in a germplasm bank aids in the identification of plants with unusual properties ranging from agronomic traits to functional and compositional traits of the seed itself. Results from this study confirm the presence of a wide variation in the thermal and functional properties of starch from several landraces of corn in the Argentinean germplasm. Thermal properties of starch measured using differential scanning calorimetry (DSC) identified several corn landraces with properties of potential commercial interest. The pasting and textural properties of gels obtained from starch of different corn landraces also exhibited considerable variability. The degree of variation in thermal and functional properties of corn reported in this study is comparable to the thermal properties of starch from several other crop species. These corn races show promise for further regeneration to create inbred lines with unusual traits. The potential for further improvement of corn races exists not only based on thermal properties, but directly for specific functional attributes as well. Correlation analyses suggest that the variability in thermal and functional attributes are a function of amylose content, granule size distribution and, possibly, differences in the structural makeup of amylose and amylopectin. The strong correlation observed between the thermal properties and pasting and textural properties will allow for the estimation of starch properties from small sample sizes.
The objective of this study was to determine the relationship between the amount and type of lipids, starch composition and structure, and storage proteins on popcorn expansion and to evaluate whether popcorns could be discriminated from other types of corn based on the protein elution parameters. Seven commercial Argentinean popcorn samples were used in the study and significant differences were observed in the popping volume of these popcorns. A significant negative correlation was observed between oleic acid and popping volume and a positive correlation was observed between linoleic acid and popping volume. Popcorn starch properties were significantly different from normal corn but no particular measured attribute of starch correlated with popping volume. α‐Zein proteins and glutelins significantly correlated with popcorn expansion volume with R2 = 0.963 and 0.744, respectively. The elution patterns of corn proteins could also be used to discriminate between different types of corn including popcorn, dent, and flint corns.
Cereal Chem. 74(1):75-78Coarse and fine kernel portions from 24 maize inbreds (six grown in two years) and four hybrids were separated by grinding and sifting. Zeins from both portions of all genotypes were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results agreed, in general, with those of a previous study in which endosperm separation was done manually, suggesting that endosperm fractionation can be done by mechanical means. Compositions of zeins, as revealed by RP-HPLC and SDS-PAGE, support the hypothesis that zeins help determine maize endosperm hardness.
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