Aim: To determine the prevalence of Campylobacter jejuni and Campylobacter coli among broilers at the time of slaughter in and around Bareilly, India. Materials and Methods:A total of 100 chicken caecal samples were screened by conventional plating in modified charcoal cefoperazone deoxycholate agar with incubation at 42°C for 48 h under microaerophilic conditions. The characteristic colonies were confirmed by morphological and biochemical characteristics and multiplex polymerase chain reaction (mPCR) assay targeting lpxA gene.Results: Out of 100 chicken caecal samples, 32 yielded isolates with typical phenotypic of Campylobacter species. The hippurate hydrolysis test found to be positive for 2 isolates, categorized as C. jejuni and negative for 30 isolates. The mPCR assay targeting lpxA gene also confirmed 2 (6.25%) isolates as C. jejuni, and 30 (93.75%) isolates as C. coli. Conclusion:The present study showed broilers to an important source of Campylobacter in the region with predominance of C. coli than C. jejuni indicating a shift in the prevalence of important species of Campylobacter. To understand the variation in pattern of occurrence of species with high prevalence of organisms, detail studies on the ecology of campylobacteriosis are suggested.

Aim:The study was carried out to determine the sensitivity and specificity of OmpA gene in Salmonella serovars through PCR. Materials and Methods:A set of primers were designed targeting the OmpA gene specific for the Salmonella and polymerase chain reaction was standardized using Salomonella Typhimurium as a positive control and as a negative control 4 non salmonella cultures such as Campylobacter coli, Arcobacter butzleri, Brucella abortus and E. coli. Sensitivity of the test was determined by serial dilution of genomic DNA of standard S. Typhimurium. The PCR standardized was used for screening 68 strains of different serovars of Salmonella. Results:The PCR developed targeting OmpA specific for Salmonella was highly specific in detection of the salmonella serovar alone and sensitivity was upto 68.8 fg. A total of 68 virulent/ natural strains of different serovars of salmonella taken up for the study were positive by OmpA based PCR. Conclusions:This study reports that, OmpA gene which is conserved among Salmonella serovars can be used for the detection of Salmonella in food or clinical samples in further studies, with high sensitivity and specificity.

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