We reported earlier that Pediococcus cerevisiae FBB-61 inhibited Lactobacillus plantarum FBB-67 in mixed species inoculation used for the fermentation of brined cucumbers. Herein, 16 isolates of the Pediococcus genus from various sources were tested for inhibitory activity against L. plantarum and other microorganisms by a seeded-agar screening technique. Only two of thq ' Paper no. 4682 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh, and paper no. 7157, Michigan Agricultural Experiment Station.
Pickling cucumbers stored at five temperatures and four relative humidities were examined for growth of eight microbial groups, the activities of two enzyme systems (pectinolytic and cellulolytic), and weight loss. Twenty-four storage tests for 6 days each were conducted during the 2-year study. In general, microbial populations of the eight groups increased rapidly at the higher temperature (>21 C) and humidity (>70%) treatments. Moisture loss of the cucumbers was rapid with combinations of high temperatures and low humidities. The results suggest that the best environmental conditions for storage or transport of cucumbers would be a combination of low temperature (10 C) with high relative humidity (about 95%). These conditions minimized undesirable microbial, enzymatic, and physical changes of stored, pickling cucumbers.
the use of propionate agar resulted in very low yeast counts in samples of plant material as compared to the populations obtained with acidified dextrose agar and synthetic agar. Acidified synthetic agar proved to be the medium of choice for the enumeration and isolation of yeasts from plant material. Counts obtained on this medium were comparable to those on acidified dextrose agar and mold growth was satisfactorily controlled because of restricted colonial development on the synthetic medium.
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