Materials Linagliptin was procured from Dr. Reddy's laboratories Ltd. Chitosan, Gelatin, sodium tri poly phosphate (STPP), glutaraldehyde, sodium bicarbonate, acetone, were procured from SD fine chemicals limited. Ethyl cellulose and HPMC E15M were procured from Yarrow Chem. Products.
A simple kinetic method has been developed based on the autoxidation of o-hydroxyphenylthiourea to disulphide, which is catalysed by trace amounts of cobalt. The reaction is monitored spectrophotometrically at pH 8.0 and cobalt is determined effectively in the range 5-50 ng ml-l. This method has been used to determine trace amounts of cobalt present in certain plant materials such as carrot roots and corn grains, in which it plays a vital role in different physiological activities.
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