The protein composition and the milk yield (morning milking) were studied in 10 Chilean Holstein–Friesian first lactation dairy cows during the spring season. In addition, the genetic variants A and B of κ‐casein (κ‐CN) and β‐lactoglobulin (β‐Lg) were also identified. The results showed that the casein content is significantly affected by the presence of B variant of κ‐CN. Moreover, positive interactions between the B variant of κ‐CN with AB of β‐Lg and the combination of A κ‐CN with AA of β‐Lg for the total protein content, were found.
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