Abstract. Knowledge about structural variations in genes and proteins relevant for fat traits is very important information to improve selection of breeding lines and preserve genetic variability in pig industry. The classical proteomics is a useful tool to separate and measure differentially expressed proteins in fat tissue of phenotypic different individual pigs. Single nucleotide polymorphisms (SNP) in the swine adipocyte fatty-acid binding protein 3 (FABP3) and leptin gene (LEP) were related to adipocyte accumulation and associated with meat quality in pigs (NECHTELBERGER et al. 2001, KULIG et al. 2001, KMIEC et al. 2003).
The aim of this study was to determine the regulatory mechanisms of molting and recrudescence via studying the micro-RNA (miRNA) expression in the oviduct of laying hens. We performed a cDNA microarray analysis in the magnum tissue from the oviduct to identify the whole miRNA profiles through the molting and recrudescence periods. A total of 35 laying hens (47-wk-old) were divided into 7 groups (0 d: a control group; 6 and 12 d: 2 molting-period groups fed on a high-zinc diet; and 20, 25, 30, and 35 d: 4 recrudescence-period groups fed on a normal diet after a 12-d period on a high-zinc diet). An miRNA co-expression network (miRCN) was generated using the differentially expressed miRNA (DEM) according to the entire data integration. The significantly co-expressed miRNA ( = 111) were highly differentially expressed from 12 to 20 d, which was a transition period between molting and recrudescence, while their expression patterns were contrary to the estrogen changes. The targets of highly connected miRNA ( = 12) indicated the significant biological pathways and gene ontology (GO) terms, such as MAPK and Wnt signaling and magnesium-ion binding, which are associated with apoptotic activities. These results suggest that the miRNA of the miRCN might play a role in the apoptotic progression of the reproductive tract during molting.
Abstract. Recently, miniature pigs have been issued for xenotransplantation studies (CHARON et al. 2000) to verify what major factors are critical matters for immune responses in human. Major histocompatibility complex (MHC) knowing for high rate of mutations and responsible for systematic immune responses is essential to understand swine immunology. Numerous studies have documented the influence of swine leukocyte antigen (SLA) genes on immune responses, organ and cell transplantation success and disease resistance (SMITH et al. 2004). The satellite region in MHC can be used to identify individuals as well as breeds, and it is important to characterize and breed unique miniature pig lines for supporting organ transplantation studies. Up to the present moment, genetic variants including repeat units (CA14-20) located between the SLA-1 and SLA-5 genes are available with GenBank acc. no. AJ131112, AP009559, AP009558, AP009556, AP009555, and CU311184, but miniature pigs have not been reported.
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