Traumatic dental injuries are often irreversible, underscoring the need for therapies that protect dental pulp cells and enhance their regeneration. We hypothesized that generation 5 poly amido amine (PAMAM) dendrimers (G5), functionalized with fluorescein isothiocyanate (FL) and αVβ3-specific, cyclic arginine-glycine-aspartic acid (RGD) peptides, will bind to dental pulp cells (DPCs) and modulate their differentiation. Dental pulp cells and mouse odontoblast-like cells (MDPC-23) (±) treated with G5-FL-RGD were analyzed via Western blot, RT-PCR, and quantitative PCR. Transcription of dental differentiation markers was as follows: Dentin matrix protein (DMP-1), dentin sialoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) as well as vascular endothelial growth factor (VEGF) all increased via the JNK pathway. Long-term G5-RGD treatment of dental pulp cells resulted in enhanced mineralization as examined via Von Kossa assay, suggesting that PAMAM dendrimers conjugated to cyclic RGD peptides can increase the odontogenic potential of these cells.

A laser-plasma-based source of relativistic electrons is described in detail, and analyzed in two dimensions using theoretical and numeric techniques. Two laser beams are focused in a plasma, one exciting a wake-field electron plasma wave while another locally alters some electron trajectories in such a way that they can be trapped and accelerated by the wave. Previous analyses dealt only with one-dimensional models. In this paper two-dimensional particle-in-cell simulations and analysis of single particle trajectories show that the radial wake field plays an important role. The simulation results are interpreted to evaluate the accelerated electron beam's properties and compared with existing devices.