Palabras clave: Microsatélite; probabilidad de exclusión; razón de verosimilitud.Keywords: Microsatellite; exclusion probability; likelihood ratio. In genetic evaluation programs, the correct identification of the individuals of the population and the certainty of their kinship relationships is indispensable. The objective of this study was to implement identity verification and paternity tests using 18 microsatellite markers, in registered Brahman cattle from Costa Rica. Genetic parameters were calculated from the analysis of 950 DNA samples (7 sires, 788 cows and 155 offspring) collected during years 2015/2016, from 13 herds with registered cattle. Average observed and expected heterozygosity were 0.67 and 0.70, respectively. The fixation index was 4%, with significant deviations of the Hardy Weinberg equilibrium in 9 of the 18 loci. All markers except ETH225 had polymorphic information content greater than 0.50. The combined probability of identity with all markers was 2.4×10 -17 for randomly selected individuals and 2.6×10 -7 for full siblings. The combined probabilities of exclusion were greater than 0.999. All these parameters presented satisfactory values for the accomplishment of tests of identity and paternity. Next, paternity confirmation tests
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