In a 2 X 3 factorial experiment with growing rats the diet varied for the iron content (5, 25, 625 ppm) and for the protein content (5, 25, 45%). The diets were fed ad libitum for 35 days. At the end of the experiment the iron content of different organs was determined. Both experimental factors and their interactions influenced the iron concentration as well as fresh weight and dry matter dependent on organ. Especially the influence of deficient protein supply and of insufficient iron supply was very marked. Dependent on the iron supply there are different responses between storage and functional compounds of iron.
In a two-factorial experiment with growing rats, protein content of diet (5, 25, 45%) and Fe supply (5, 25, 625 mg/kg diet) were changed. Both factors as well as their interactions influenced growth (p less than 0.001). The growth was reduced especially by deficient protein supply but although by inadequate iron supply and in a smaller degree by an excessive protein content of the diet. Hematological values as hemoglobin content, counts and volume of erythrocytes, hematocrit, MCH and MCHC - measured after 35 days of the experiment - were influenced by both factors and their interactions, too (p less than 0.001). Again deficient protein supply and insufficient Fe supply have the marked effects. Referring life weight as well as hematological parameters, the deficient protein supply was - independent of Fe supplementation - the limited factor, whereas in the cause of higher protein content (25, 45%) an insufficient Fe supply has negative effects.
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