The effect of variation in berry size on berry composition was studied in irrigated Syrah/R99 grapevines, located in a temperate area of South Africa. Berries from 45 clusters, sampled from both sides of the canopy (east and west), were weighed to create four categories: 1. less or equal to 1.5 g; 2. between 1.51 g and 2.00 g; 3. between 2.01 g and 2.50 g; 4. more than 2.50 g. Berry physical characteristics were determined and total anthocyanins and seed flavonoids were analysed by spectrophotometry and anthocyanin profiles by HPLC. The ratio of skin weight:berry weight did not change with increasing berry size, but the ratio of seed weight:berry weight increased. For total anthocyanins, varying results were obtained if values were expressed in mg/berry, mg/g skin, mg/kg grape and mg/cm 2 skin. The quantity of total anthocyanins changed positively with increasing berry weight if expressed in mg/berry and remained almost similar if expressed in mg/cm 2 of skin. In contrast, total polyphenols decreased with berry weight if the values were expressed in mg/kg grape and in mg/g skin. Total anthocyanins expressed in mg/kg grape and in mg/g skin were correlated positively to the number of berries as well as to the total skin surface in one kilogram of grape. In this study, the largest berries seemed to have lower quality characteristics. In order to obtain better wine quality, it seems important to reduce berry weight and, in general, berry size variability of Syrah. The obtainment and continuity of a particular wine style may be affected by berry variability.
The effect was studied of partial defoliation (33% and 66%) on the sugar and acid accumulation and pH in grapes of Vitis vinifera L. cv. Cabernet Sauvignon. Although the total soluble sugar (TSS) in grapes of partially defoliated vines was significantly higher than that of non-defoliated vines in some cases, no significant differences were generally found. No significant differences in total titratable acidity (TT A) were found between treatments. The timing of defoliation had no effect on TSS in grapes, whereas TT A tended to be higher the earlier partial defoliation was commenced. In general, 33% and 66% defoliated vines respectively produced approximately 33% and 200% more TSS and TT A in the fruit per cm 2 leaf area than non-defoliated vines. No significant differences between defoliation treatments were found on a per gram dry berry mass or per berry basis for glucose and fructose or tartaric and malic acid. However, 66% defoliated vines had significantly less soluble solids in berries per shoot, which was probably caused by a lower total berry mass per shoot. Although no significant differences in sugar composition could be found between defoliation treatments, tartaric acid levels tended to be higher and malic acid levels lower as a result of partial defoliation. Partial defoliation had no effect on the accumulation patterns of sugars and acids. Glucose dominated in berries at veraison, with fructose dominating at ripeness. The highest total tartaric and malic acid concentrations occurred at pea size. Malic acid content decreased rapidly from veraison, whereas the decrease in tartaric acid was not pronounced. Must pH was not affected by partial defoliation. The results seem to suggest that the general metabolism of vines was favourably changed by partial defoliation, mainly in terms of a more favourable source: sink ratio, more efficient photosynthesis, and an improved canopy microclimate.
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