Abstract. Glucocorticoids (GCs) modulate the synthesis of many pro-inflammatory cytokines and influence multiple transduction pathways. GCs negatively or positively influence the transcription factors of their target genes. All of these transcription signals are closely connected to cancer survival or death. We investigated the action of dexamethasone (DEX) on head and neck cancer cell lines. When SNU-1041 and SNU-1076 were treated with DEX, the cell lines showed different patterns of responses. DEX inhibition of cell growth depended on concentration in SNU-1041, but not in SNU-1076. Furthermore, DEX suppressed vascular endothelial growth factor (VEGF) secretion from SNU-1041, but not from SNU-1076. We explored the mechanism that explains these distinct differences. After DEX treatment, the differences of NF-κB (p65), glucocorticoid receptor and p-AKT were not observed between the cell lines. However, phospho-signal transducer and activator of transcription 3 (STAT3) decreased in SNU-1041 only. Moreover, STAT3 inhibition using si-RNA suppressed VEGF secretion. When STAT3 was overexpressed after DEX treatment, the level of VEGF in the culture media was restored. Taken together, we suggest that p-STAT3 can be a mediating factor which regulates VEGF secretion in the DEX treatment. Because the relationship between the three molecules DEX, STAT3 and VEGF is scarcely known, our findings clarified one of the signaling pathways of DEX, which is often used in clinical conditions.
Poster Presentations
P163Objectives: 1) Investigate the adverse events (AEs) associated with surgery for early-stage oral cancer. 2) Characterize surgical procedure and its related AEs.Methods: Based on the National Institute of Health/National Cancer Institute Common Terminology Criteria for Adverse Events (v4.0), patient self-reported AEs post-surgery in a pan-Canadian multi-center phase III randomized controlled surgical trial (the COOLS trial) were captured during the follow-up visits.
Background and ObjectivesZZThis study aimed to evaluate the clinical efficacy of sentinel node centered selective neck dissection in patients with early stage tongue cancer (T1T2N0). Subjects and Method Lymphoscintigraphy was performed for 12 patients, subsequently followed by sentinel node centered selective neck dissection. The location of the sentinel node, pathological confirmation of node metastasis, and follow-up recurrence were analyzed. Results In total, 19 sentinel lymph nodes were identified. Of these, 18 were located in levels I to III, and one in level IV. After surgery, 3 patients (25%) were diagnosed with neck node metastasis: two experienced sentinel node metastasis and one experienced skipped metastasis. During follow-up, 3 of the 12 patients (25%) experienced recurrence. Conclusion The recurrence of lymph node could be covered with supraomohyoid neck dissection, which indicates that it has superiority over sentinel node centered selective neck dissection in preventing recurrence in T1T2N0 tongue cancer patients. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
POSTERSResults: Fifteen patients (12 males) seen between 1990 and 2009, with a median age of 56.7 years. Seven patients had the diagnosis of moderately differentiated neuroendocrine carcinoma, and 8 were diagnosed as small cell neuroendocrine carcinoma. Multimodal treatment was established in 13 (92.8%) patients. Eight patients underwent surgical treatment. Neck node metastases developed in 6 patients (42.5%), and distant metastases occurred in 12 patients (80%). Cause of death was distant metastases in 11 patients (73.3%). At last follow-up, only two patients were alive. The 3-year OS and DSS were, respectively, 35.7% and 28.5%.Conclusion: Laryngeal neuroendocrine malignant neoplasms (LNMN) are aggressive malignancies with a high frequency of distant metastasis. The optimal therapy for patients with LNMN remains a subject of debate.
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