-Apis mellifera is an important pollinator but is sometimes associated with adverse effects on natural ecosystems. We surveyed pairs of disturbed and undisturbed sites across three biomes in South East Australia. We used pheromone lures to trap drones, genotyped the drones to infer the number of colonies within flight range and then estimated colony densities using synthetic sampling distributions. Estimated colony densities ranged from 0.1 to 1.5 colonies km −2 and significantly lower in agricultural land relative to national parks. We suggest that colony density may be lower in disturbed than 'natural' areas due to the reduced availability of nest sites and possibly pesticide usage. Because the number of colonies recommended for adequate pollination is 100-1000 colonies km −2 , there are insufficient bees to provide optimal crop pollination in the areas we surveyed.feral honey bees / colony density / microsatellite haplotypes / population size

An epidemiological study of the nematode parasites of the eastern grey kangaroo (M. giganteus) was made in an enclosed reserve north of Melbourne, Victoria, Australia. Adult and juvenile kangaroos were collected at approximately 6-weekly intervals and blood, faeces and the entire gastrointestinal tract was obtained. The nematodes present were Globocephaloides trifidospicularis, Rugopharynx australis, R. rosemariae, Pharyngostrongylus kappa, Cloacina spp., Strongyloides sp., Macropostrongylus baylisi, Paramacropostrongylus toraliformis, Alocostoma clelandi, Labiostrongylus spp., Macroponema comani and Macropoxyuris. The majority of nematodes had a seasonal fluctuation. Acquisition commenced in late summer and reached a peak in midwinter before declining in spring. G. trifidospicularis caused considerable mortality in juvenile kangaroos and there was a strong relationship between rising numbers of this species and falling plasma protein, haemoglobin concentration and haematocrit values. This species can cause heavy mortality in juvenile kangaroos in enclosed populations when winter feed is depleted and when the small animals with no fat reserves experience maximum cold stress. Other parasites present included Progamotaenia ewersi, P. festiva, P. macropodis, Triplotaenia fimbriata, T. undosa, Eimeria wilcanniensis, E. macropodis, E. hestermani and E. marsupialum.

In a survey in eastern Australia, 104 Macropus giganteus and 40 M. fuliginosus were examined at autopsy for helminths. 32 species of nematodes (28 strongyloids 2 oxyuroids and 2 filarioids) and 6 species of anoplocephalid cestodes were found. Most species occurred in both hosts. The most prevalent and numerous nematode in both species was Rugopharynx australis which reached numbers up to 290 000 in M. fuliginosus. Pharyngostrongylus kappa was also common and widespread but occurred only in M. giganteus. The most striking stomach worms were Labiostrongylus kungi and L. bipapillosus which occurred in both hosts. Various Cloacina spp. were found, some specific to one host, others occurring in both. Other species of stomach nematodes were relatively uncommon. The parasites of the small intestine were 2 species of Globocephaloides, 2 of Triplotaenia and 2 of Progamotaenia. 2 other species of the latter genus occurred in the bile ducts. The large intestine harboured 6 species of nematodes, including Macropoxyuris spp. which were very numerous in both hosts. The 2 species of filariids found (both in Macropus giganteus) were Breinlia mundayi (new host record) in the abdominal cavity and Dirofilaria roemeri in the connective tissues.

A total of 115 Macropus rufus was collected at bimonthly intervals at Menindee, New South Wales, Australia, in 1976 and 1977 and examined for parasites and pathological changes. The animals came from 2 populations, one free-ranging and the other a high-density, enclosed population in Kinchega National Park. No differences in species of helminths present, in prevalence or intensity of infection were noted between the 2 populations. The stomachs were infected with Labiostrongylus longispicularis (prevalence 87.8%), Rugopharynx australis (98.2%), Zoniolaimus spp. (81.6%), Cloacina spp. (71.1%), Papillostrongylus labiatus (47.4%) and Filarinema spp. (92.2%). Hypodontus macropi (93.0%) was the dominant species among the 2 species of cestodes and 3 of nematodes found in the intestine. Progamotaenia festiva (in 74.3%) was found in the bile ducts where it caused mild cholangitis. Only 2 nematode species, F. flagrifer and H. macropi, caused gross and histological lesions. Apart from L. longispicularis none of the helminths showed seasonal variations in prevalence or numbers. Data on worm numbers in relation to host age and on site distribution in the host are presented. The various haematological parameters measured showed no evidence of adverse effects by the parasites, and corticosteroid levels were judged to be within the normal range. The helminth faunas of 9 M. fuliginosus and one M. robustus, grazing sympatrically with M. rufus inside the park are listed. ADDITIONAL ABSTRACT: The parasites reported from 115 red kangaroos (Macropus rufus) in New South Wales in 1976-77 include Tracheomyia macropi (Frogg.), which infected the trachea in 54.5% of the kangaroos [see RAE/B 4, p. 122].

It is often assumed that the density of feral honey bee colonies in Australia is sufficient to provide adequate pollination services to the many agricultural crops that require pollination. In contrast, there is concern that the density of feral colonies is sufficiently high to have inimical effects on Australian biota. For both these reasons, it is desirable to have robust estimates of the density of feral honey bee colonies in Australian landscapes. In this study, we mated four to five queens with wild drones at disturbed and undisturbed sites in three of the major ecosystems in Victoria Australia and examined the paternities of worker offspring to estimate the density of feral colonies within mating range of the test queens. We show that the density of feral colonies differs little with land use (cleared or uncleared) and is similar across the state. Our data suggest that the density of feral colonies is probably insufficient to provide adequate pollination of agricultural crops and that neither land use nor local climate variation is a major factor determining density. Finally, our data suggest that the mating range sampled by test queens is significantly greater than previously assumed.