ABSTRACT. Kisspeptins, the product of the KISS1 gene, play an essential role in the regulation of reproductive functions, acting primarily at the hypothalamic level of the gonadotropic axis. We detected polymorphisms of the goat KISS1 gene in 723 individuals from three goat breeds (Xinong Saanen, Guanzhong, and Boer) by DNA pooling, PCR-RFLP, and DNA sequencing methods. We cloned the promoter sequence of this gene and found it to share high similarity with that of the bovine KISS1 promoter. Six TATA boxes were found in the goat KISS1 promoter region. Two novel SNPs (g.2124T>A and g.2270C>T) were identified in the intron 1 of the KISS1 gene of all three goat breeds. The three goat breeds were in Hardy-Weinberg disequilibrium at g.2124T>A and g.2270C>T loci. The g.2124T>A and g.2270C>T loci were closely linked in the three goat breeds (r 2 > 0.33). The g.2124T>A and g.2270C>T SNPs were significantly associated with litter size, and the C1 female goats had a larger litter size than did those with the other genotypes. These results extend the spectrum ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4308-4316 (2013) Effect of KISS1 gene mutations on goat litter size 4309 of genetic variation of the goat KISS1 gene, which contributes to our knowledge of goat genetic resources for breeding programs.
ABSTRACT. Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex ® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an offladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify this locus, monogenic amplification, gene cloning, and genetic sequencing were performed. Sequencing analysis demonstrated that the fragment size of the Penta D-OL locus was 469 bp and the core sequence was [AAAGA] 21 , also called Penta D-21. The rare Penta D-21 allele was found to be distributed among the Zhuang population from the Guangxi 15097 "Off-ladder" allele at the Penta D locus ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15096-15101 (2015) Zhuang Autonomous Region of China; therefore, this study improved the range of DNA data available for this locus and enhanced our ability for individual identification of gene loci.
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