No abstract

Six murine monoclonal antibodies to Leishmania tropica major have been prepared and the properties of these antibodies studied. Two (WIC 79.3 and 79.7) were L. tropica major species-specific and bound to promastigote cell surfaces, to parasitized macrophages, but not isolated amastigotes. No evidence was found for the production of antibody to the antigenic determinants recognized by WIC 79.3 or 79.7 during L. tropica major infections in mice and hamsters. One antibody (WIC 79.1) bound to sub-cellular organelles of Leishmania species but to a different sub-cellular organelle of Trypanosoma cruzi. Two others bound to the flagellum, one (WIC 79.2) to all Leishmania species, T. cruzi and Trypanosoma brucei, the other (WIC 79.4) only of L. tropica major and L. donovani species. One antibody (WIC 79.5) was directed against an unknown internal antigen found in all Leishmania species and T. cruzi.

Using the Sovereign phacoemulsification unit with the WhiteStar system during 2-stab-incision bimanual microphacoemulsification with a bare 19-gauge aspiration needle in human cadaver eyes, a wound burn could not be produced at the highest energy settings unless all flow into the eye and all aspiration were occluded. These settings were well beyond clinically applicable conditions and provide evidence that microphacoemulsification can be performed safely with the WhiteStar system.