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A facile and economical hydrothermal method was adapted to synthesis spinal Zn 2 SnO 4 nanoparticles with cubic structure. The crystallography and optical properties of as synthesised nanoparticles have been studied using XRD (X-ray diffraction) and (UV-Vis)UV-Visible spectroscopy. The morphology of the nanoparticles has been revealed via FESEM (Field emission scanning electron microscopy). The synergetic antibacterial effect of Zn 2 SnO 4 nanoparticles against gram positive and gram negative pathogenic bacterial strains were investigated. The results suggest that Zn 2 SnO 4 nanoparticles have a potent antibacterial property against both gram classes of bacteria suggesting that nanoparticles of Zn 2 SnO 4 can be used as a bactericidal agent to prevent and control the spreading and persistence of infectious diseases.