A review is given of the techniques that have been used in the construction of dynamic tension-testing machines. The problems involved in obtaining reliable tensile stress-strain curves at high strain rates are discussed. A new apparatus, designed to give accurate stress-strain curves in tension at strain rates of the order of 1000 per second, is then described. The theoretical basis of the test method is indicated, and some experimental results obtained with the apparatus are presented, both for materials that exhibit a definite yield point and for those that do not.
The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentation equilibrium in the analytical ultracentrifuge and model-independent SEDFIT-MSTAR analysis across a range of loading concentrations. The change in the weight average molar mass M
w with loading concentration was consistent with a monomer-dimer equilibrium. Overlap of data sets of point weight average molar masses M
w(r) versus local concentration c(r) for different loading concentrations demonstrated a completely reversible equilibrium process. At the clinical infusion concentration of 5 mg.mL−1 all glycopeptide is dimerized whilst at 19 µg.mL−1 (a clinical target trough serum concentration), vancomycin was mainly monomeric (<20% dimerized). Analysis of the variation of M
w with loading concentration revealed dissociation constants in the range 25-75 μM, commensurate with a relatively weak association. The effect of two-fold vancomycin (19 µg.mL−1) appears to have no effect on the monomeric enterococcal VanS kinase involved in glycopeptide resistance regulation. Therefore, the 30% increase in sedimentation coefficient of VanS on adding vancomycin observed previously is more likely to be due to a ligand-induced conformational change of VanS to a more compact form rather than a ligand-induced dimerization.
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