Purified mRNAs coding for # and K human immunoglobulin polypeptides were translated in vitro and their products were characterized. The p-specific mRNAs, derived from both human lymphoblastoid cells (GM607) and from a mouse-human somatic cell hybrid secreting human p chains (aD5-DH11-BC11), were copied into cDNAs and inserted into the plasmid pBR322. Several recombinant cDNAs that were obtained were identified by a combination of colony hybridization with labeled probes, in vitro translation of plasmidselected p mRNAs, and DNA nucleotide sequence determination. One recombinant DNA, for which the sequence has been partially determined, contains the codons for part of the C3 constant region domain through the carboxy-terminal piece (155 amino acids total) as well as the entire 3' noncoding sequence up to the poly(A) site of the human p mRNA. The sequence A-A-U-A-A occurs 12 nucleotides prior to the poly(A) addition site in the human p mRNA. Considerable sequence homology is observed in the mouse and human p mRNA 3' coding and noncoding sequences.
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