Sugarcane lignocellulosic biomass (trash, depithed bagasse, whole bagasse from mills) and wheat straw were evaluated as substrates in five treatments for the edible mushroom Pleurotus ostreatus cultivation. Sugarcane substrates were characterized according to TAPPI and AOAC methods to determine their chemical composition. The substrate mixture were sterilized and placed in polyethylene bags before being inoculated with the Pleurotus ostreatus spawn. Colonizing and fructification periods and biological efficiency were analyzed. The results showed colonizing and fructification periods similar to the control group with trash (RAC) treatments and RAC/BI (Trash and bagasse from mills), biological efficiency of 106% and 103%, respectively. Edible mushroom Pleurotus ostreatus showed 15.2 and 21.3% of raw protein, 12.8% of crude fiber, 5.8 and 6.7% of ash, and 40 and 45% of carbohydrates. Mushroom cultivation is an efficient biological process of food protein recovery from sugar cane lignocellulosic biomass.
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