We have examined and determined the preliminary crystallization conditions of more than one hundred proteins, pep tides, and nucleic acids in our laboratory. This presentation will review this experience as an effmt towards providing insight into the an-ay of techniques one may utilize towards deteil"l1ining preliminary crystallization conditions of macromolecules as well as paths for the optimization. Various sparse mal:!i' protocols were utilized which screened a range of salts, polymers and organic solvents over a pH range of2 to 12. G1id screens utilizing single and multiple precipitant systems of varying concentration over broad pH ranges were also utilized. Once piimary crystallization variables were deteil"l1ined, a number of optimization screens were developed and utilized to fine tune the solution conditions for crystal growth. The protocols for these screens will be reviewed and presented. To manage the considerable amount of crystallization data generated dming expe1in1enation, a relational database was developed and is now utilized in the design, formulation, execution, doc=entation, and review of crystallization experiments. With an ongoing accumulation of data from crystallization experiments, the database is becoming an increasingly impmtant tool in the design and implementation of screening and optimization strategies while at the same time improving expeiimentation speed and efficiency. The features of the crystallization database will be presented, concluding vvith suggested areas of exploration for c1ystallization screening. MS16.01.02 POLYTHER.J.vlAL SCREENJNG AJ.'ID PRODUCTION METHODS FOR l\llACROMOLECULAR CRYSTALLI-ZATION.
No abstract
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