No abstract

No abstract

Wedelia trilobata (L.) Hitch is a member of the family Asteraceae, with attractive yellow flowers borne singly at the end of each stem. The work was conducted to investigate the antioxidant, antimicrobial and DNA protecting ability of the methanol extract of W. trilobata flower. Antioxidant properties were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis 3-thylbenzthiazoline-6-sulphonic acid (ABTS) assay. The methanol extract and standard ascorbic acid showed antioxidant activity with IC 50 value of 90 µg/ml and 60 µg/ml respectively in DPPH radical scavenging assay. The in vitro antibacterial screening was evaluated by disc diffusion method against three Gram-positive and three Gram-negative human pathogenic bacteria. The extract showed zones of inhibition of 10-16 mm against different strains. The phenolic content of the extract was 250 gallic acid equivalence (GAE in µg) which was measured by Folin-Ciocalteu method. The extract was tested for pTZ57R/T plasmid DNA protection against hydroxyl radicals as evidenced by DNA fragmentation assay.

No abstract

Abstract.A series of 4-aryl-N-(4-pheny-thiazol-2-yl)-5,6-dihydro-4H-1,3,4-oxadiazine-2-carboxamides were synthesized by condensing 4-aryl-5,6-dihydro-4H-1,3,4-oxadiazine-2-carboxylic acid with 2-amino-4-aryl-thiazole derivatives. The newly synthesized molecules were characterized by spectral analysis and subjected to antioxidant and DNA damage inhibition studies.

Abstract:Background: Wedelia trilobata (L.) Hitch (WT), commonly known as yellow dots or creeping daisy, is a shrub possessing potent biological activities, and is traditionally used a medicinal plant in Ayurveda, Siddha and Unani systems of medicines, and it has also been tried against leukemia cell line MEG-01. In the present study, purification and screening of the plant was done for bioactive compounds in methanolic extract of WT for apoptotic and antileukemia activity. Materials and methods:The methanolic extract of WT was initially purified through thin layer chromatography (TLC) and screened for the apoptotic and anti-leukemia activities. The positive band of TLC was subjected to silica gel column chromatography for further purification and the fractions obtained from it were screened again for antileukemia activity through thymidine uptake assay and apoptotic activity by DNA fragmentation, nuclear staining and flow cytometry assays. The fraction with positive result was subjected to HPLC for analysis of bioactive components. Results: Out of many combinations of solvents, the methanol and dichloromethane combination in the ratio 6:4 has revealed two bands in TLC, among which the second band showed positive results for apoptotic and anti-leukemic activities. Further purification of second band through silica gel chromatography gave five fractions in which the 3

Novel Pyrazole Integrated 1,3,4-Oxadiazoles: Synthesis, Characterization and Antimicrobial Evaluation. -Among the synthesized oxadiazole derivatives, compound (IIIh) emerges as an effective antimicrobial agent. -(NINGAIAH, S.; BHADRAIAH*, U. K.; DODDARAMAPPA, S. D.; KESHAVAMURTHY, S.; JAVARASETTY, C.; Bioorg. Med. Chem. Lett. 24 (2014) 1, 245-248, http://dx.

A series of pyrazoline amidoxime and pyrazolyl‐1,2,4‐oxadiazole derivatives are synthesized and screened for their in vitro antioxidant, antimicrobial and antiinflammatory activities.