The effect of a palatal acrylic appliance and/or infection with Candida albicans on the permeability of rat palatal epithelium has been investigated. Although normal rats, or rats inoculated with Candida albicans but without a prosthesis, had a barrier to perfused lanthanum, some low-molecular-weight proteins were able to pass through the epithelium.When infection was established by inoculation of Candida albicans under an acrylic plate, the epithelium became permeable to perfused lanthanum. Polyacrylamide gel electrophoresis of perfusates showed that a selective permeability to proteins was retained in such animals. Removal of acrylic plates from infected animals resulted in healing and restoration of the barrier to lanthanum.Oral candidosis and in particular chronic atrophic candidosis is a common condition in denture wearers [6]. It usually affects the palatal epithelium and is caused by the fungus Candida albicans [7,8]. The histopathological features of chronic atrophic candidosis have been described [6] and are largely non-specific. The palatal tissue is chronically inflamed and hyperplastic, with occasional fungal hyphae penetrating as far as the inferior border of the stratum granulosum. With the exception of some clinical and histopathological observations there is little information available on the effect of C. albicans on palatal tissues. Healthy palatal epithelium has barrier properties which prevent the ingress of water and water-soluble compounds, and toxins or other deleterious substances [14]. The permeability barrier can be demonstrated ultrastructurally by the use of electron-dense materials such as lanthanum nitrate [23]. Hyperplasia, induced by turpentine or mild trauma has been shown to affect the permeability of oral epithelium [23]. No reports have been traced concerning the effects of infection on barrier function.The aim of this investigation was to study the effect of C. albicans on the barrier properties of palatal epithelium. A rat model for the induction of palatal candidosis
This facilitates the rapid production of reproducible thin sections. With freeze-dried, embedded hypertrophic cartilage, the morphology was similar to that seen using aqueous fixatives even when no additional electron density is in-mitochondria cytoplasm 7 Unosmicated 8 4,
SUMMARY The preparation of biological tissues for electron microscopy by rapid freezing retains the original localization of ions and molecules. A reproducible freezing regime was established by quenching tissues in liquid propane according to the method of Elder et al. (1981). Tissue was thereafter freeze dried in a custom built freeze drying device with a liquid nitrogen cooled stage to prevent ice recrystallization during drying. The device was also designed to allow the vacuum embedding of tissue in low temperature resin such as Lowicryl® and polymerization in situ. This paper describes the design of the device and an example of its use in the freeze drying of cartilage. The results show that minimal ice damage occurs to the chondrocytes and that intracellular organelles are clearly visible. The regime described may prove a useful and pragmatic alternative to cutting tissue in the frozen state. Translocation of elements is unlikely except perhaps in the case of very labile elements such as Na and K, but this remains to be fully elucidated.
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