The effects of lead, iron, copper, and zinc ions on 8-aminolevulinic acid dehydratase from red blood cell haemolysates in humans, both in the absence and presence of plasma proteins, have been investigated. 8-aminolevulinic acid dehydratase (ALAD) was not found to be a specific indicator of blood lead concentrations since it was also inhibited by copper and activated by zinc. Plasma protein protected the enzyme from both inhibition and activation. ALAD activity was found to be an indicator of the total metal ion concentration in the blood and was therefore considered to be of doubtful value in screening large populations for increased lead absorption.
Renal Function after Leptospirosis-Simpson et al. MEDICAL JOURNAL 473 instances six, serotypes. In only two were the maximum titres against the serotypes responsible for the original infection.
DiscussionOur results suggest that the long-term prognosis after the acute renal lesion of leptospirosis is good. Thus in the British patients all the results of renal function tests were normal except for a slightly reduced creatinine clearance in one man; as the serum creatinine level and other results in him were normal the significance of this isolated finding is doubtful. Another man developed hypertension, but renal damage did not appear to be the cause. One group of Gurkha patients had normal creatinine clearances but all did not concentrate urine adequately, while in the others these findings were reversed; since each abnormality was confined to one group it seems likely that these discrepancies reflect incomplete dehydration and incomplete urine collection respectively.As intravenous pyelograms were performed in only four patients, and renal biopsy in none, we
An optimized assay for alkaline phosphatase (EC 3.1.3.1) is reported. A discrete analyzer, the DuPont Automatic Clinical Analyzer (aca), was used for this study. The assay is based on results of response-surface experimental co-optimization techniques, and response is enhanced over the present aca assay. A key feature is the incorporation of zinc ions, both to fully optimize the assay and to reduce the sensitivity of measured activity to zinc-binding impurities in the buffer, 2-amino-2-methyl-1-propanol. In addition, a simple technique is described for measuring relative concentrations of zinc-binding impurities in this buffer. These features should be considered in the design of any assay for alkaline phosphatase that is based on p-nitrophenyl phosphate as substrate and 2-amino-2-methyl-1-propanol as buffer.
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