The polymerase chain reaction (PCR) is a powerful method that allows enzymatic amplification of race target nucleic acid sequences. It has been applied to the amplification of viral genomes from paraffin-embedded pathology specimens. However, interpretation of negative results requkes amplification of a housekeeping gene such as b-actin. In the present study we used specific oligonucleotide primers previously designed to amplify both the genomic DNA and the mRNA transcript from paraffin-embedded tissue. These products have predicted sizes of 250 BP and 154 BP, respectively, but our results showed that PCR amplifcation only (without reverse transcription) unexpectedly generated the 1544'
The (27)(28)(29)(30). Furthermore, the scid-related defects in DNA repair and V(D)J recombination were also complemented by transfecting V3 cells with yeast artificial chromosomes (YACs) containing the
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