Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
In this paper we show that the adoption of a simple weighted ordering heuristic strategy to find fixed shortest path routes in a topology can improve the load-balancing and, consequently, the network performance. We compared our fixed routing strategies against k-shortest paths (k-SP) fixed-alternate routing strategy, for three different mesh topologies considering fixed grid networks (FGN) and elastic optical networks (EON). The results show that our simple fixed routing strategies can improve the network performance for an optical network system, even compared with the fixed-alternate routing, and always using the shortest path. Results also show that such easy strategies act mainly in longer routes, increasing their probability of use, and balancing the link load distribution.
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