We sought to determine whether sperm donor selection could be improved by using the hyaluronan binding assay (HBA) score to predict live birth rates (LBRs) in donor insemination treatments. Thirty donor samples were included: five fresh samples to assess whether HBA score is affected by cryopreservation and 25 frozen samples from donors whose sperm gave rise to known live births, divided into three groups, with success rates ≥50%, <50-33% and <33%, to examine whether HBA score correlates with LBRs. Correlations with semen analysis parameters, as well as donor demographic factors, known causes of patient subfertility and/or the type of treatment provided were also assessed. There was no significant difference in the mean HBA score pre- and post-cryopreservation (p = 0.998) nor in the HBA score of the three different outcome groups (p = 0.89). HBA score was not significantly affected by other semen analysis parameters (r=0.394, p = 0.127) or any of the other examined factors. This was the first study to investigate HBA score for cryopreserved donor samples with known LBRs. HBA score was not correlated with LBRs in this small pilot study. Larger studies are required to validate HBA score as a tool for sperm donor selection and predictor of LBRs.

Successful fertilisation is one of the key steps determining success of assisted conception. Various factors including sperm or oocyte pathology and environmental factors have a significant impact on fertilisation rates. This systematic review is aimed to evaluate the existing evidence about factors affecting fertilisation and strategies to improve fertilisation rates. A literature search was performed using Ovid MEDLINE ® (Jan 1950-April 2016), EMBASE (Jan 1950-April 2016), Ovid OLDMEDLINE ®, Pre-MEDLINE (Jan 1950-April 2016) and the Cochrane Library. Relevant key words were used to combine sets of results and a total 243 papers were screened. Only qualitative analysis was performed, as there was major heterogeneity in study design and methodology for quantitative synthesis. Factors affecting fertilisation were divided into sperm- and oocyte-related factors. The methods to improve fertilisation rates were grouped together based on the approach used to improve fertilisation rates. Optimising laboratory condition and procedural effects in techniques is associated with improved fertilisation rates. Various techniques are described to improve fertilisation rates including assisted oocyte activation, physiological intracytoplasmic sperm injection (PICSI) and intracytoplasmic morphologically selected sperm injection (IMSI). This review highlights the promising strategies under research to enhance fertilisation rates. Adequately powered multicentre randomised trials are required to evaluate these techniques before considering clinical application.

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