Fresh vegetables and peeled sugar-cane serves as an essential component of a healthy diet, but despite their benefit they are prompt to bacterial contamination. The bacteriological quality of four types of fresh vegetables and peeled sugar-cane obtained from selected markets in Zaria, Nigeria was determined using standard microbiological method. The fresh vegetables, peeled sugar-cane, water and hand-swab samples were collected from four different locations namely; Samaru, Sabon-gari, Tudun-wada and Zaria-city. The total bacteria plate counts ranged from 3.3 x 10 6 cfu/g to 1.6 x 10 7 cfu/g with Tudu-wada having the highest bacteria load in lettuce. The coliform count of fresh vegetables and peeled sugar-cane varies from 1.3 x 10 4 to 8.6 x 10 5 cfu/g in the entire markets with Tudu-wada having the highest value in cucumber, the counts were obviously above specified Standard of 10 5 cfu/g and 10 3 cfu/g (ICMSF, 1978). Pathogenic bacteria isolated include; Citrobacter fruendii, Escherichia coli, Enterobacter spp., Klebsiella. spp., Shigella spp., Serratia spp., Cronobacter sakazaki, Staphylococcus aureus, Pseudomonas spp., and others Staphylococci spp.. This study showed that, fresh vegetables and peeled sugar-cane are contaminated with pathogenic bacteria making them unsafe for human consumption; hence fresh produce should be pre-treated thoroughly, so as to reduce the risk of food-borne outbreaks.
This study was conducted to compare the specificity and sensitivity of GeneXpert MTB/Rif and Lowenstein-Jensen (LJ) Proportion methods of detecting rifampicin-resistant Mycobacterium tuberculosis among Acid Fast Bacilli (AFB) positive patients attending the Directly Observed Treatment (DOT) Centre of Aminu Kano Teaching Hospital Kano. A total of 150 AFB positive samples were collected and processed according to the guideline given by National TB and Leprosy Control Program (2015) and WHO (2012), The result revealed that rifampicin-resistant Mycobacterium tuberculosis (RR-TB) from the samples was very high; 66.7% and 60.8% for GeneXpert MTB/Rif and Lowenstein-Jensen (LJ) Proportion methods respectively. Cohen’s Kappa (interrater reliability) statistical analysis indicated a substantial agreement between GeneXpert and LJ Proportion specificity and sensitivity (Kappa value = 0.73). Keywords: Mycobacterium tuberculosis, GeneXpert MTB/Rif, Lowenstein-Jensen (LJ) Proportion, Rifampicin resistance.
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