Background and objective: Exosomes are small vesicles secreted from many cell types. Their biological effects largely depend on their cellular origin and the physiological state of the originating cells. Exosomes secreted by mesenchymal stem cells exert therapeutic effects against multiple diseases and may serve as potential alternatives to stem cell therapies. We previously established and characterized human leukocyte antigen (HLA) haplotype homo (HHH) dental pulp cell (DPC) lines from human wisdom teeth. In this study, we aimed to investigate the effect of local administration of HHH-DPC exosomes in a mouse model of periodontitis.Methods: Exosomes purified from HHH-DPCs were subjected to particle size analysis, and expression of exosome markers was confirmed by western blotting. We also confirmed the effect of exosomes on the migration of both HHH-DPCs and mouse osteoblastic MC3T3-E1 cells. A mouse experimental periodontitis model was used to evaluate the effect of exosomes in vivo. The morphology of alveolar bone was assessed by micro-computed tomography (μCT) and histological analysis. The effect of exosomes on osteoclastogenesis was evaluated using a co-culture system. Results: The exosomes purified from HHH-DPCs were homogeneous and had a spherical membrane structure. HHH-DPC exosomes promoted the migration of both human DPCs and mouse osteoblastic cells. The MTT assay showed a positive effect on the proliferation of human DPCs, but not on mouse osteoblastic cells. Treatment with HHH-DPC exosomes did not alter the differentiation of osteoblastic cells. Imaging with µCT revealed that the exosomes suppressed alveolar bone resorption in the mouse model of periodontitis. Although no change was apparent in the dominance of TRAP-positive osteoclast-like cells in decalcified tissue sections upon exosome treatment, HHH-DPC exosomes significantly suppressed osteoclast formation in vitro. Conclusions: HHH-DPC exosomes stimulated the migration of human DPCs and mouse osteoblastic cells and effectively attenuated bone loss due to periodontitis.
Background: 3M microfoam™ surgical tape (3ST: 3M Japan Limited) is used for pressure wound control of medical equipment. It is cushioned and can be fitted to any body part. Here we investigated whether 3ST prevents nasal pressure injury associated with nasotracheal intubation (NTI). Methods: We conducted a prospective, randomized double-blind study, enrolling 63 patients aged 20 to 70 years, who underwent general anesthesia with NTI. They were divided into 2 groups; those treated with 3ST (group S ; n = 31) and control (group C ; n = 31). After NTI and before securing the nasotracheal tube, a 35 × 25 mm 3ST was used to protect the nasal wing in group S , and the nasotracheal tube was fixed in place after NTI without protection in group C . The primary outcome was the presence or absence of nasal pressure injury after extubation. The Chi-Square test was used to assess the association between the 2 categorical variables. Results: Nasal pressure injury was observed in 7 and 19 patients from groups S and C , respectively, representing a significant difference between the 2 groups (24.1% vs 67.8%, P = .001). Remarkably, none of the patients developed ulcers. Conclusion: 3ST prevents nasal pressure injury associated with NTI.
BACKGROUND ː 3M Microfoam™ Surgical Tape (3ST: 3M Japan Limited) is a surgical tape used for pressure wound control of medical equipment and has characteristics of being cushioned and fitting to any body part. Herein, we investigated whether 3ST prevents nasotracheal intubation (NTI)-associated nasal pressure injury. METHODS ː We conducted a prospective, randomized double-blind study, enrolling 63 patients aged 20–70 years, who were subjected to general anesthesia with NTI and divided into two groups; those treated with 3ST (group S; n = 31) and control (group C; n = 31). After NTI and before securing the nasotracheal tube a 35 × 25 mm 3ST was used to protect the nasal wing in group S, and the nasotracheal tube was fixed in place after NTI without protection in group C. The primary outcome was the presence or absence of nasal pressure injury after extubation. Chi-Square test was used to assess the association between the two categorical variables. RESULTS ː Nasal pressure injury was observed in 7 and 19 patients from groups S and C, respectively, representing a significant difference between the two groups (24.1% vs. 67.8%, P = 0.001). None of the patients developed ulcers. CONCLUSIONS ː 3ST prevents NTI-associated nasal pressure injury.
Von Recklinghausen’s disease is characterized by skin pigmentation, multiple neurofibromatosis, and osseous changes. In the management of anesthesia, a variety of pathologies need to be taken into account. This case describes the perioperative management of a patient with Recklinghausen’s disease suspected difficulty in airway management.
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