We investigated whether juveniles of the nocturnal fish jundiá (Rhamdia quelen) and the diurnal fish Nile tilapia (Oreochromis niloticus) are able to chemically communicate stress to conspecifics. Groups of 8 fish were reared in tanks under recirculated water (water exchanged among all the tanks) for each species. Fish were handled in half of the tanks (stressor fish) and whole-body cortisol concentrations were compared among handled fish, non-handled fish exposed to water from the handled fish, and non-handled control fish held with no water communication. For each treatment cortisol concentrations were determined before exposure to the stressor (basal levels) and after 1, 2, 4, 8, and 24h. Basal levels of cortisol confirmed fish were unstressed in the beginning of the experiment. Cortisol was increased in the stressor fish 1h after handling. Fish receiving water from the stressor fish increased cortisol levels later (2h after the stressor fish were handled). As the isolated control group maintained cortisol levels unchanged throughout the experiment, we concluded that some chemical factor was released by the stressed fish in the water and thus stressed the conspecifics. This pattern was similar for both unrelated species, thus suggesting that this communication might have evolved earlier in fish and reinforcing the biological value of this kind of information.
The purpose of this study was to verify the effect of dietary Ca 2+ on the growth and survival of silver catfish fingerlings (Rhamdia quelen) exposed to different water pH (5.5, 7.5 and 9.0). Silver catfish fingerlings were randomly placed in a thermoregulated water re-use system with twelve 250 L-tanks, two 1000 L-biofilters and a 2000 L-reservoir with a medium flow of 3.84 L min )1 tank. Stocking density was 0.16 fingerlings L )1 . To prepare the treatment diets, the control diet (0.8 g kg )1 Ca 2+ ) was supplemented with CaCO 3 to yield experimental diets with 6.4, 9.5 and 23.9 g kg )1 Ca 2+ . There were three replicates/treatments. Survival was more than 93.9% in all treatments. Exposure of silver catfish fingerlings to alkaline or acid water reduced growth, and this effect was not ameliorated by dietary Ca 2+ supplementation. Moreover, when fingerlings were maintained in water with pH 7.5, the best dietary Ca 2+ range for silver catfish fingerling growth was 0.8-6.4 g kg )1 .
KEY WORDS
correspondência.
RESUMO
O trabalho foi conduzido com o objetivo de testar diferentes fontes protéicas em dietas práticas para alevinos de jundiá (Rhamdia quelen). Foram realizados dois experimentos
Lipids include some of the most important nutrients that affect the survival and growth of fishes in their early life stages. Lipid deficiency prior to spawning may significantly reduce egg production, hatchability and the number of surviving larvae. In this study, we investigated the effects of isocaloric diets containing 80, 140 and 200 g kg À1 of lipids (lipid source, soy oil) for 90 days. The following data were collected at 0, 45 and 90 days: final weight; length; conditioning factor; hepatosomatic, gonadosomatic and visceral fat indices; total plasma protein; testosterone; 17ß-estradiol; free amino acids; and ovary and muscle fatty acid profiles. Additionally, fecundity, oocyte production, egg morphometric parameters, and larval and postlarval growth were evaluated. Results were not statistically different for husbandry and biochemical parameters, but visceral fat content increased with increase in dietary lipid levels. The fatty acid profiles and composition differed among dietary treatments. The egg diameter and area were significantly low in fish fed the 200 g kg À1 lipid diet, thereby hindering growth, survival and weight compared to those in postlarval fish (P < 0.05). The best reproductive rates were obtained when using diets containing 80-140 g kg À1 total lipids.
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