RESUMOA grande variabilidade patogênica do fungo Colletotrichum lindemuthianum e a ampla distribuição geográfica em regiões produtoras de feijão no Brasil vêm causando sérios problemas a produtores e melhoristas. Assim, o objetivo deste trabalho foi realizar um levantamento de raças de Colletotrichum lindemuthianum provenientes de cultivos de feijoeiro comum no Oeste do Estado do Paraná, região de destaque na produção brasileira. Após coleta das vagens com antracnose e a desinfecção superficial, com hipoclorito de sódio, fragmentos das lesões foram transferidas para meio BDA e estreptomicina, seguidas de incubação por oito dias a 28 ºC. Para a seleção de isolados o micélio do fungo foi repicado duas ou três vezes em placa de Petri contendo o mesmo meio. Após a incubação obteve-se uma suspensão de esporos, pelo método de diluições, a qual foi ajustada para a concentração de 1,2 x 10 6 esporos mL -1 . Aplicou-se a suspensão na primeira folha trifoliolada das doze cultivares diferenciadoras. Após 10 dias avaliaram-se os sintomas, adotando-se a escala de notas de um a nove. Dentre os 61 isolados avaliados, identificaram-se as raças 52, 65, 81, 83, 321 e 337. As raças 52, 83 e 337 foram observadas pela primeira vez em cultivos de feijoeiro comum no Oeste do Estado do Paraná, o que comprova o aumento da variabilidade do patógeno na região e a necessidade de monitoramento periódico.Palavras-chave: Antracnose; cultivares diferenciadoras; raças. ABSTRACTThe great pathogen variability of the fungus Colletotrichum lindemuthianum and its widespread distribution across several common bean regions in Brazil, particularly in the southern and southeastern regions, is causing serious problems for producers as well as breeders. The purpose of this paper was the identification of Colletotrichum lindemuthianum races in common bean fields in the western part of Paraná state, one of the leaders in the Brazilian production. Anthracnose-infected pods were collected and the surface disinfected with sodium hypochlorite. Lesion fragments were transferred to PDA medium containing streptomycin and incubated for eight days at 28 ºC. For selection of the isolates the mycelium of the fungus were seeded two or three times on Petri dishes with the same medium. After incubation a spore solution was obtained by the dilution method, adjusted to a concentration of 1.2 x 10 6 spores mL -1 . The suspension was applied on the first trifoliote leaf of the 12 differentiating cultivars. The symptoms were evaluated after 10 days, on a 1 to 9 scale. Among the 61 evaluated isolates, the races 52, 65, 81, 83, 321, and 337 were identified. The races 52, 83 and 337 were observed for the first time in common bean fields in western Paraná that demonstrates increase in the variability of the pathogen in the region and the need of a periodic monitoring.
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