We studied the levels and patterns of genetic variability in eight species of native fi shes from the Valdivia River Basin (Región de los Rios, Chile), with the aim to estimate microevolutionary differences in species with similar ecological and historical conditions. The species considered were Diplomystes camposensis, Percilia gillissi, Galaxias platei, G. maculatus, Basilichthys australis, Percichthys trucha, Aplochiton zebra and A. taeniatus. We compared genetic diversity indexes both intra and inter-zones, and genetic structure, based on sequences of the control region for different sections along the river. In addition, we recovered relationships among haplotypes and their spatial distribution for each species,
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