An experiment was conducted to evaluate the effect of a low-pathogenic H6N2 avian influenza A viral strain (LPAIV strain H6N2) on subsequent (after 3 days) vaccination with a lentogenic avian paramyxovirus serotype 1 strain La Sota (APMV-1 strain La Sota) in guinea fowl. The effects were monitored by detection of the presence of viruses in cloacal and oropharyngeal samples, as well as by the presence of humoral immune response. The obtained results were compared to birds with monoinfections. Replication and virus shedding of LPAIV strain H6N2 from the cloaca and the oropharynx were established, while APMV-1 La Sota was reisolated only from the oropharynx. The reisolation of LPAIV strain H6N2 was similar in both monoinfection and co-infection. The dynamics of virus replication of APMV-1 strain La Sota was affected in the beginning of the co-infection, later occurrence of the peak which matched the period of decline of LPAIV strain H6N2 reisolates. The LPAIV strain H6N2 and APMV-1 strain La Sota co-infection did not exert any influence on humoral immune response to both viruses.
The haemagglutination inhibition (HI) test is used for testing 1258 blood sera from four avian species (hens and broilers, turkeys, partridges and geese) for detection of post infection antibodies against avian paramyxovirus -2 (АРМV-2). The samples were collected from 14 regions in Bulgaria from fowl reared in 27 farms (commercial laying hens, commercial broilers, commercial laying turkeys and game breeding station with partridges) -15, 9, 2, 1 samples, respectively. Another 11 samples were obtained from backyard poultry (laying hens, laying turkeys, laying geeses) -8, 2, 1 birds respectively. Positive results from tests were established in sera from backyard geese, from commercial broilers, commercial laying hens, and partridges (75%, 12.96 %, 7.81 % and 5.17 % respectively). In studied farms with hens and broiler chickens, positive samples from broilers predominated (88.89 %), while the prevalence on laying hen farms was 66.67 %. Positive samples were detected in 10 (71.43 %) out of the 14 surveyed regions. Antibody titres varied from 1:4 (log 2 2) in hens, broilers and geese, 1:16 (log 2 4) in partridges and attained 1:32 (log 2 5) in broilers, 1:64 (log 2 6) in geese and partridges and 1:128 (log 2 7) in laying hens. Among geese and partridges, samples with titres 1:16 (log 2 4) were predominating -60 % and 66.67 %, followed by broilers with titres 1:8 (log 2 3) -37 % and hens with titres 1:4 (log 2 2) -47 %.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.