Nicotine degrading bacteria was isolated from crude unprocessed tobacco from local vendor of Solapur city. Using medium containing whole tobacco extract as the sole carbon and nitrogen source. One purified isolate with a high capacity for tobacco utilization was characterized for 16s rRNA and identified as Stenotrophomonas maltophilia by phylogenetic analysis and on morphological and biochemical features. The optimal culture condition of strain Stenotrophomonas maltophilia for tobacco utilization is pH 6.5 and 30-34 °C and 0.5 g/ L−4.5g/L of nicotine concentration in the inorganic salt medium, which is basically consistent with utilization of nicotine by the isolate. The growth and nicotine degradation of the isolate suggested that strain Stenotrophomonas maltophilia could utilize nicotine from the extract as sole source of carbon, nitrogen and energy. The thermal denaturation test indicated that the G + C content of the DNA was 55.11 mol%.
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