AbstrakPenelitian ini bertujuan mengetahui tingkat kematian udang vaname yang diinfeksi bakteri Pseudomonas sp. Bakteri Pseudomonas sp. diisolasi dari sedimen tambak udang. Uji patogenitas menggunakan udang vaname (7-9 gram) yang diinfeksi bakteri Pseudomonas sp. dengan kepadatan 10 2 , 10 4 , 10 6 , 10 8 sel/ml secara intramuscular sebanyak 0,1 ml/ekor. Perlakuan kontrol diinfeksi menggunakan NaCl fisiologis sebanyak 0,1 ml/ekor. Tingkat mortalitas dan gejala klinis diamati setap 6 jam pada 24 jam pertama dan selanjutnya selama 24 jam hingga 10 hari. Hasil yang diperoleh dari uji patogenitas bakteri Pseudomonas sp. pada udang vaname menunjukkan tingkat kematian sebesar 0% pasca infeksi. Hal ini menunjukkan bahwa bakteri Pseudomonas sp. tidak menyebabkan kematian pada udang vaname. Gambaran gejala klinis yang ditunjukkan berupa hepatopankreas berwarna hijau kehitaman dengan tekstur yang padat dan menunjukkan kenampakan yang sama dengan perlakuan kontrol. Warna tubuh udang vaname setelah diinfeksi bakteri Pseudomonas sp. menunjukkan gambaran yang sama dengan kontrol yaitu berwarna putih transparan. Karapas dan ekor udang vaname setelah diinfeksi menunjukkan tidak adanya kerusakan yang disebabkan oleh infeksi bakteri Pseudomonas sp., gambaran tersebut menunjukkan kenampakan yang sama dengan perlakuan kontrol. Hal ini menunjukkan bahwa bakteri Pseudomonas sp. tidak patogen terhadap udang vaname dan infeksi bakteri Pseudomonas sp. tidak berpengaruh terhadap perubahan morfologi makroskopis dan tingkah laku. AbstractThis research aims to determine the mortality of vannamei shrimp infected by Pseudomonas sp. bacteria isolated from sediment of shrimp pond. Pathogenicity test was by using vannamei shrimp (7-9 grams) with a density of 10 2 , 10 4 , 10 6 , 10 8 cells/ml intramuscularly. The control treatment was infected by using a physiological NaCl of 0.1 ml. The rate of mortality and clinical symptoms were observed for the first of 6 hours for 10 days. The results from the pathogenic test of Pseudomonas sp. in vannamei showed that 0% post infection of mortality rate. This was indicating that the Pseudomonas sp. bacteria did not cause death in vannamei shrimp. The clinical symptoms showed in the form of blackish-green hepatopancreas with a dense texture. That clinical symptom had the same appearance as the control treatment. The color of vannamei body showed a white color and so with the control treatment. Carapace and tail showed no damage to treatment and control treatment. This indicated that the bacteria Pseudomonas sp. did not cause death to vannamei shrimp and bacterial infections. Pseudomonas sp. did not affect morphological changes in macroscopic and behavior.
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