Background: Silver nanoparticles (SNPs) play important role in the field of optics and electronics and also as a novel antibacterial agents. Here, we report a simple and green method for the biosynthesis of SNPs using aqueous leaf extract of Origanum majorana and Citrus sinensis as a novel bio source of cost-effective, non-hazardous reducing, and stabilizing agents. A 3 mM solution of silver nitrate was prepared. Five milliliter aqueous leaf extract was slowly added to 20 ml silver salt solution (3 mM) with constant stirring. No noticeable color change was observed. The solution was then heated in domestic microwave for variable time intervals. The intense brown colored solution was obtained on 1 min heating with O. majorana and 5 min heating with C. sinensis extract. The intense brown color indicated the formation of SNPs. The antibacterial activity of synthesized SNPs was investigated.Results: SNPs were rapidly synthesized using aqueous leaf extract of O. majorana and C. sinensis on microwave irradiation. Formation of SNPs was confirmed by the change in color from yellowish green to brown and absorption maximum around ~420 and 410 nm due to surface plasmon resonance of SNPs. They were also characterized by other physical-chemical techniques like Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope coupled with X-ray energy dispersive spectroscopy, and high-resolution transmission electron microscopy. TEM analysis showed the presence of feather-shaped NPs in O. majorana and spherical as well as cubical-shaped NPs in C. sinensis-mediated SNPs. The synthesized SNPs showed significance antibacterial activity against two human pathogenic strains.
Conclusion:The SNPs were synthesized using leaf extract of plants. This synthesis method is nontoxic, eco-friendly, and a low-cost technology for the large-scale production. The SNPs can be used as a new generation of antibacterial agents.
The research work performed and described
here is in regard to
synthesis of dextrin-sodium alginate based hybrid hydrogel film. It
showed the maximized percentage swelling at 1145% after optimization
of all reaction parameters. It was further functionalized with a fluorophore,
i.e., riboflavin, to introduce luminescent behavior to the film. The
RDSA film can not only remove but also sense the copper and nickel
ions in the surroundings very sensitively up to 0.4445 μM and
0.2571 μM, respectively, which is in the desired range of EPA
limits. The RDSA film could detoxify the 5 μM copper and nickel
ion concentrated solution to an extent of 95.54% and 91.30%, respectively.
The adsorption followed the Flory–Huggins model along with
second-order kinetics specifically for higher metal ion concentrations.
The detection and adsorption occur as a result of complex formation
between the film and metal ion. The intraparticle diffusion mechanism
is of two steps and occurs through the outer surface to the inner
pores. The ions can also be desorbed with EDTA to reuse the film for
further recovery of the metal ions. It can be done for multiple cycles,
and finally the film can be degraded biologically when it is of no
use. It is due to natural materials being used for the synthesis.
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