This study evaluated cryopreserved homografts (Group 1) and porcine heterografts decellularized with deoxicholic acid (Group 2), implanted in the right ventricular outflow tract of juvenile sheep. Two groups with four animals in each were used and all animals survived with good outcome. Animals were sacrificed 90 or more days after surgery (90-150 days). On the third and fifth postoperative months they were submitted to echocardiographic examination with normal function and appearance observed for both groups. Explants were evaluated through histological analysis, atomic spectrophotometry and radiological examination. Calcium content was higher in the cusps of cryopreserved homografts, despite an otherwise similar macroscopic appearance between grafts of both groups. Decellularized heterografts were progressively repopulated by autologous cells suggesting some regenerative ability and longer durability than conventional homografts.
Objective: The aim of this study was to retrospectively evaluate the first eight years of activities of the Human Heart Valve Bank of Hospital de Caridade da Irmandade da Santa Casa de Curitiba (BVCHSC), analyzing aspects related to procurement, processing, storage and distribution of homologous cardiovascular grafts.Method: Initial donor screening was achieved according the national guidelines for human organ procurement, besides specific BVCHSC criteria. Hearts were obtained from multiple organ donors, non-beating heart donors and heart transplant recipients, with total ischemic times under 48 hours. Donor ages varied from neonates up to 60 years for aortic valves and 65 years for pulmonary valves. The dissected grafts had their dimensions measured and their morphology evaluated, using a classification system with category 0 (discarded), 1 (minimal morphological alterations) and 2 (perfect). The incidence and microorganisms responsible for organ contamination were determined, as was the efficiency of the decontamination solution. Aspects related to graft distribution were also analysed. Results: From September 1996 to February 2005, 1059hearts from 19 Brazilian states were received at BVCHSC. From these, 977 (92.3%) were from brain-death donors. A total of 2105 grafts were processed and of the aortic and pulmonary grafts that were analysed, 783 were in category 2, 697 in category 1 and 186 were discarded due to morphological abnormalities. In total, 433 received grafts were contaminated and the sterilization solution was efficient in 330 cases. 571 (27.1%) grafts were rejected during some phase of the processing, mainly due to contamination and morphological abnormalities. A total of 1338 grafts were distributed to 74 health institutions and were more commonly used for aortic valve replacement (529), correction of congenital heart diseases (478) and during the Ross procedure (272).Conclusions: The activities of the BVCHSC during the initial eight years were satisfactory, fulfilling the proposed goals.Descriptors: Tissue banks. Transplantation, homologous. Cryopreservation. Tissue preservation. 399COSTA Cardiovasc Surg 2005; 20(4) , MTBA ET AL -Analysis of the inicial eight years of activities of the Human Heart Valve Bank of the Hospital de Caridade da Irmandade da Santa Casa de Misericórdia de Curitiba Braz J
Atualmente, na constante busca de minimizar o tempo de intemação hospitalar e melhorar a recuperação no pós-operatório, várias altemativas cirúrgicas têm sido aventadas. Iniciamos o uso da estemotomia parcial como acesso para diversos tipos de operações cardíacas. O presente trabalho tem como objetivo apresentar a evolução hospitalar dos pacientes operados através desta técnica. No período de novembro de 1996 a março de 1997, estudamos 51 pacientes operados através da esternotomia parcial. A idade média foi de 46,8 anos, sendo 26 pacientes do sexo feminino. Os procedimentos mais realizados foram o tratamento cirúrgico valvar e as revascularizações miocárdicas. O acesso utilizado para os pacientes com lesões valvares foi a esternotomia parcial em "T" invertido no segmento superior do esterno; para outras lesões, uma esternotomia em "T" no segmento inferior e, pela dificuldade técnica imposta nas re-operações e nos procedimentos múltiplos, utilizou-se uma terceira variação, que foi a esternotomia parcial em "H" deitado, estendendo-se no corpo esternal do primeiro ao quarto espaço intercostal. O comprimento médio da incisão de pele foi de 9,9 cm. Foram realizadas 19 incisões em "T", 17 em "T" invertido e 15 em "H" deitado. O tempo médio de ventilação mecânica foi de 2,8 horas, de permanência na UTI de 31,5 horas e de internação hospitalar de 5,9 dias. Não houve complicação diretamente relacionada com o acesso e em apenas 1 caso foi necessária a conversão para a esternotomia total. Analisando a evolução destes 51 pacientes, pudemos concluir que a esternotomia parcial é um acesso seguro para o tratamento cirúrgico de diversas cardiopatias, isoladas, associadas ou re-operações. Traz um resultado estético favorável e facilita a recuperação no pós- operatório, devendo fazer parte do repertório de todo o cirurgião cardiovascular.
Recently, in the constant search for shortening hospital stay and emproving postoperative recovery, many surgical alternatives have been used. We started the use of a partial sternotomy as an approach to different types of cardiac operations. This paper aims to show the hospital outcome of patients submitted to this technique. From November 1996 to March 1997, 51 patients who underwent partial sternotomy were studied. The mean age of the patients was 46.8 years with 26 females. The most common procedures were valve surgeries followed by myocardial revascularization. The approach used in the patients with valve pathologies was a partial sternotomy in an inverted "T" incision in the superior segment of the sternum, for other pathologies a sternotomy in a "T" incision in the inferior segment was the choice and due to technical difficulties imposed by reoperations and in multiple procedures a third variation, partial sternotomy in an "H" incision was used. In the latter, the sternum was cut from the first to the fourth intercostal spaces. The average size of the skin incision was 9.9 cm. There have been 19 "T" incisions, 17 inverted "T" incisions and 15 "H" incisions. The average assisted ventilat...
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