Mutants of Saccharomyces cerevisiae that require cAMP for growth have been isolated. Some of the mutants isolated were deficient in adenylate cyclase activity and mapped at a locus, cyrl, located near the centromere of chromosome X.Growth of cells carrying the cyrl mutation was arrested at the GI phase of the yeast cell cycle in the absence of cAMP. The cyrl mutation was suppressed by a secondary mutation designated bcyl. The bcyl mutation bypassed the need for cAMP for growth. The bcyl mutants had extremely low levels of cAMP-binding protein and cAMP-dependent protein kinase but produced a high level of cAMP-independent protein kinase. The results indicate that cAMP is an essential factor for yeast cells to proceed through the cell cycle via the activation of protein kinase.
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