In a previous paper,1} we reported the isolation and identification of an alkalophilic bacterium, Bacillus sp. GIR621 , for the pretreatment of pectinaceous wastewater discharged from citrus processing facilities. This strain extracellularly produced a small amount of e^do-pectate lyase (endo-FATE, EC 4.2.2.2). Here, we further studied this strain with the aim of moreefficient pretreatment of the wastage, i.e., the accelerated degradation of the pectic substances contained. This strain was mutagenized with Af-methyl-N-nitro-7Vnitrosoguanidine and then the resultant PATE-mass
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