Background Marbofloxacin (MAR), a second-generation fluoroquinolone, is used in veterinary medicine in the form of tablets. It has a broad spectrum of action, low toxicity, and limited development of bacterial resistance. The analytical methods available in the literature become more important since MAR in tablets does not have a monograph in official compendiums. Objective Thus, the purpose of this review is to display them according to the analyzed matrix and place them according to the conditions used in the scope of green analytical chemistry, in addition to discussing possible gaps and opportunities for the development of new methods. Results MAR, being an antimicrobial, presents both physical-chemical (93%) and microbiological (7%) methods in the literature. Among the methods found, 53% are for analysis of food matrices using preferably HPLC and TLC-MS. 27% are for analysis of biological matrices and 20% are for analysis of pharmaceutical matrices, and in both HPLC is preferably used. Conclusions Therefore, there is still a gap in the literature in relation to other options of analytical methods for the analysis of MAR, which are faster, such as microbiological turbidimetry, sustainable, such as miniaturized methods, and ecologically correct, such as those that do not usetoxic organic solvents. Highlights A review of the status of analytical methods available in the literature for assessing the quality of MAR and MAR-based products were exhibited and discussed, as well as new opportunities for analysis according to green analytical chemistry.
Background Marbofloxacin (MAR) is an antimicrobial for veterinary use and it does not have a monograph in an official compendium for the pharmaceutical form in tablets. Objective In this context, the objective of this work was to develop and validate an effective, eco-friendly and indicative of stability method by UV for quantitative evaluation of MAR in tablets. Method Purified water + ethanol (80:20, v/v) as a diluent, quartz cubette and 296 nm were used. Results The method was linear in the range of 6 – 21 µg/mL (0.9994) with detection and quantification limits of 0.39 and 1.18 µg/mL, respectively, selective in the comparison of standard and sample, precise in intraday (RSD 1.20%), interday (RSD 1.68%) and between analysts (RSD 3.26%) levels, exact with average recovery of 100.39% and robust against small changes in wavelength, diluent concentration and the use of the ultrasound device. The forced degradation test (sample solutions prepared in 0.1 M HCl, 0.1 M NaOH and neutral conditions and kept at 60 °C during 2 hours, and exposure to UV 254 nm lamp at ambient temperature during 2 hours) showed that the proposed method is able to assess the susceptibility of MAR. Conclusions So, the method can be reliably applied in routine analysis of MAR in tablets as well as being indicative of stability, eco-friendly, effective, accessible and following the principles of green analytical chemistry.
Background: The pharmaceutical industry is in constant development, aiming to increase its portfolio, optimizing time, product quality and efficacy along with patient safety. The main goal of developing and validating an analytical method is to achieve a balance between costs and risks within the wide array of technical possibilities in order to assure that the method is capable of meeting its expectations, ensuring effective quality control. Objective: The objective of this mini-review is to discuss the analytical aspects of development and validation for analysis of pharmaceutical products, focusing on the complete evaluation package in a systematic way to demonstrate the optimal performance of the method. Methods/Results: Validation results are obtained following strict protocols, typically starting with the assessment of selectivity/specificity parameters, followed by parameters such as linearity and precision. Moreover, accuracy, detection limit, quantification limit and method robustness are also evaluated. Conclusion: This paper may serve as a guide for the pharmaceutical-chemical laboratory, conceptualizing quality and current analytical needs, according to Green Analytical Chemistry, for the development and validation of reliable methods, ensuring clarity to the analyst and assisting in decision making.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.