Technology-facilitated abuse or ‘tech abuse’ in intimate partner violence (IPV) contexts describes the breadth of harms that can be enacted using digital systems and online tools. While the misappropriation of technologies in the context of IPV has been subject to prior research, a dedicated study on the United Kingdom’s IPV support sector has so far been missing. The present analysis summarises insights derived from semi-structured interviews with 34 UK voluntary and statutory sector representatives that were conducted over the course of two years (2018–2020). The analysis identifies four overarching themes that point out support services’ practices, concerns and challenges in relation to tech abuse, and specifically the Internet of Things (IoT). These themes include (a) technology-facilitated abuse, where interviewees outline their experiences and understanding of the concept of tech abuse; (b) IoT-enabled tech abuse, focusing on the changing dynamics of tech abuse due to the continuing rise of smart consumer products; (c) data, documentation and assessment, that directs our attention to the shortcomings of existing risk assessment and recording practices; and (d) training, support and assistance, in which participants point to the need for specialist support capabilities to be developed within and beyond existing services.<br /><br />Key messages<br /><ul><li>UK statutory and voluntary support services do not feel well equipped to respond to tech abuse.</li><br /><li>Shortcomings in documentation and assessment practices make it difficult to estimate the full scale and nature of tech abuse.</li><br /><li>Tech abuse training and other support mechanisms are needed to amplify the UK sector’s ability to assist IPV victims/survivors.</li></ul>
The analysis of proteins and protein complexes by cross-linking mass spectrometry (XL-MS) has expanded in the past decade. However, mostly used approaches suffer important limitations in term of efficiency and sensitivity. We describe here a new workflow based on the advanced use of the trifunctional cross-linker NNP9. NNP9 carries an azido group allowing the quantitative and selective introduction of a biotin molecule into cross-linked proteins. The incorporation is performed by click-chemistry using an adapted version of the enhanced filter-aided sample preparation (eFASP) protocol. This protocol, based on the use of a molecular filter, allows a very high recovery of peptides after enzymatic digestion and complete removal of contaminants. This in turn offers the possibility for one to analyze very large membrane proteins solubilized in detergent. After trypsin digestion, biotinylated peptides can be easily enriched on monoavidin beads and analyzed by LC-MS/MS. The whole workflow was developed on creatine kinase in the presence of detergent. It led to a drastic improvement in the number of identified cross-linked peptides (407 vs 81), compared to the conventional approach using a gel-based separation. One great advantage of our enhanced cross-linking mass spectrometry (eXL-MS) workflow is its high efficiency, allowing the analysis of a very low amount of material (15 μg). We also demonstrate that higher-energy collision dissociation (HCD) outperforms electron-transfer/higher-energy collision dissociation (EThcD) in terms of number of cross-linked peptides identified, but EThcD leads to better sequence coverage than HCD and thus easier localization of cross-linking sites.
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