In vitro multiplication via organogenesis in squash. This study was carried out at Univiersidad autonoma of chapingo, mexico, in 2004, to develop appropriate protocols for the induction of direct organogenesis and to characterize in vitro regeneration capacity of two squash (cucurbita pepo L.) genotypes: the 'Brujita' variety and the 'round Zucchini' variety (coat-less seed), and to develop acclimation procedures for plantlets obtained in vitro. murashige and skoog (1962) culture media, supplemented with 1,0 mg/l of 6-benzylaminopurine (BaP), was used. after three weeks of induction in the culture media, explants of the two varieties regenerated sprouted. adventitious shoots were sub-cultured to allow their rooting and elongation in murashige and skoog media without the BaP hormone. The acclimation of the plantlets was carried out in plastic trays containing Peat moss® as substrate and covered with transparent plastic simulating a "micro-greenhouse". In vitro multiplication rate differed among genotypes; the coat-less seed 'round Zucchini' variety had 3,2 plantlets per bud (explant) and the 'Brujita' variety had 2,7, counted every twenty two days. The acclimation process used yielded 90% survival the 'Brujita' variety plantlets, and 76% survival in the coat-less seed 'round Zucchini' variety.
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