Densely packed polysaccharide brushes consisting of α-d-glucose residues were grafted from modified silicon substrates.
Potato phosphorylase was herein used to grow linear polysaccharide
chains from silicon tethered maltoheptaose oligosaccharides using
glucose-1-phosphate as donor substrate. The combined use of potato
phosphorylase and Deinococcus
geothermalis branching enzyme resulted in a hyperbranched brush coating as the
latter one redistributes short oligosaccharides from the α(1–4)-linked
position to the α (1–6)-linked position in the polysaccharide
brush. The obtained grafting density of the brushes was estimated
on 1.89 nm–2 while the thickness was measured with
ellipsometric techniques and determined to be between 12.2 and 20.2
nm.
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