Understanding the catalysis of oxidative reactions by TAML activators of peroxides, i. e. iron(III) complexes of tetraamide macrocyclic ligands, advocated a spectrophotometric procedure for quantifying the catalytic activity of TAMLs for colorless targets (kII′, M−1 s−1), which is incomparably more advantageous in terms of time, cost, energy, and ecology than NMR, HPLC, UPLC, GC‐MS and other similar techniques. Dyes Orange II or Safranin O (S) are catalytically bleached by non‐excessive amount of H2O2 in the presence of colorless substrates (S1) according to the rate law: −d[S]/dt=kIkII[H2O2][S][TAML]/(kI[H2O2]+kII[S]+kII′[S1]). The bleaching rate is thus a descending hyperbolic function of S1 : v=ab/(b+[S1]). Values of kII′ found from a and b for phenol and propranolol with commonly used TAML [FeIII{o,o′‐C6H4(NCONMe2CO)2CMe2}2(OH2)]+ are consistent with those for S1 (phenol, propranolol) obtained directly by UPLC. The study sends vital messages to enzymologists and environmentalists.
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