Background: There are few studies exploring the antioxidant properties of the latex C-serum of the Hevea brasiliensis latex and their possible use for therapeutic application. Objective: This study investigated the in vitro antioxidant activity of the latex C-serum of H. brasiliensis and assessed the cytotoxicity of this fraction in two human cell lines derived from normal tissue (MRC-5 and CCD 1059sk. Chemical characterization was also assessed. Methods: Latex from H. brasiliensis was centrifuged and the C-serum phase was tested for in vitro free radical scavenging assays, such as hydroxyl and nitric oxide radicals, hydrogen peroxide and total antioxidant capacity by the phosphomolibdate method. Latex C-serum was also investigated for cytotoxic properties by the MTT test. Latex C-serum was chemical characterized by Infrared Spectroscopy Fourier Transform (FTIR). Results: Latex C-serum effectively scavenged hydroxyl and nitric oxide radicals and hydrogen peroxide and presented potent antioxidant activity. The results were compared to ascorbic acid, a standard antioxidant. The cytotoxicity evaluation of latex C-serum demonstrated cell line dependency and for CCD 1059sk cells derived from skin fibroblasts, latex C-serum was not cytotoxic. FTIR showed the presence of proteins in latex C-serum. Conclusion: Latex C-serum from Hevea brasiliensis presented strong antioxidant potential and these results openthe possibility of further studies aiming the use of latex C-serum as treatment for skin diseases related to reactive oxygenspecies or skin preventive pharmacological formulations.
Objective: The aim was to evaluate the association between the MTHFR 677C>T (rs1801133) variant with susceptibility and severity of peripheral arterial occlusive disease (PAOD) and levels of homocysteine (Hcy). Subject and Methods: A case-control study enrolled 157 patients with PAOD and unrelated 113 controls. The severity and anatomoradiological categories of the PAOD were assessed by Fontaine classification and Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC), respectively. The MTHFR 677C>T was genotyped using real-time polymerase chain reaction and Hcy serum levels were determined using chemiluminescence microparticles assay. Results: The PAOD patients showed higher Hcy than controls but the values did not differ according to Fontaine and TASC categories. The MTHFR 677C>T variant was not associated with PAOD, the clinical stage, and the anatomoradiological categories. However, patients carrying the TT genotype (recessive model) or CT+ TT genotypes (dominant model) presented higher levels of Hcy than those carrying other genotypes. In conclusion, the T allele of MTHFR 677C>T variant was associated with hyperhomocysteinemia in PAOD patients, but not in controls. Conclusion: Our data suggested a possible interaction between MTHFR 677C>T variant and the presence of other genetic, epigenetic or environment factors associated with PAOD on modulation of the Hcy metabolism.
The aim of this study was to evaluate the association between the MTHFR 677C > T (rs1801133) genetic variant with susceptibility and severity of peripheral arterial occlusive disease (PAOD) and with serum levels of homocysteine (Hcy). This case-control study enrolled 157 patients with PAOD attended at University Hospital of Londrina, and unrelated 113 healthy individuals from Southern Brazil. The clinical severity of the PAOD patients was assessed by Fontaine classification and anatomoradiological categories by Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC). The MTHFR 677C > T was genotyped using real-time polymerase chain reaction. The PAOD patients showed higher Hcy than controls but the Hcy did not differ according to Fontaine and TASC categories. Patients carrying the TT genotype (recessive model) or CT + TT (dominant model) presented higher levels of Hcy than those carrying other genotypes. In conclusion, the T allele of MTHFR 677C > T variant was associated with hyperhomocysteinemia in PAOD patients, but not in controls. Moreover, this variant was not associated with the clinical stage and the anatomoradiological categories of PAOD. Our data suggested a possible interaction between MTHFR 677C > T variant and the presence of other genetic, epigenetic and environment factors associated with PAOD on modulation the metabolism of Hcy.
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