Strategies to tailor the Sn-Beta-catalysed methyl lactate process are identified by kinetic and mechanistic insights.

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BackgroundBesnoitiosis is caused by different species of intracellular protozoan parasites belonging to the family Sarcocystidae and affecting multiple host species worldwide. Including B. besnoiti, ten species are described infecting animals. Among ungulates, Besnoitia bennetti infects horses, donkeys and zebras and was described in Africa and in the USA where donkey besnoitiosis is considered as an emerging disease.Case presentationA two-year-old male donkey was purchased in May 2016 in poor body condition (cachexia, alopetic areas and pruritus mainly on neck and head) by the present owner in Le Roeulx (Belgium) from a milk producing donkey farm in Frasnes-lez-Buissenal (Belgium). Shortly after its purchase and shearing, the donkey presented with crusts, hyperkeratosis (both flanks and neck) anorexia and cachexia. A treatment with phoxim was given with no improvement. A cutaneous biopsy of hyperkeratotic skin was performed in July. It showed a perivascular eosinophilic infiltrate with a large thick walled cyst located in the dermis containing numerous bradyzoites. This was highly suggestive of besnoitiosis. Several skin biopsy samples were obtained for qPCR analysis and confirmed the presence of Besnoitia spp. DNA. Further laboratory diagnosis tests were performed (western blot and rDNA sequencing) confirming Besnoitia bennetti aetiology for the male. For the female, the punch-biopsy, haematology and qPCR were negatives but the western blot showed the presence of antibodies directed to Besnoitia spp. Further clinical examination performed in August highlighted scleral pinhead sized cysts (pearl) in the right eye and between nares. Another ten-year-old female donkey purchased in France and sharing the same accommodation showed a good clinical condition, but a thorough clinical examination showed the presence of numerous cysts on the inner face of upper labial mucosa. A daily treatment based on sulfamethaxzole and trimethoprim (Emdotrim 60% Mix®, 30 mg/kg) was given orally and some improvement was noticed.ConclusionThis is the first evidence of Besnoitia bennetti infection (Protozoa: Apicomplexa) in donkeys (Equus asinus) in Belgium.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2993-3) contains supplementary material, which is available to authorized users.

Sn-Beta zeolites are among the most promising catalysts for the conversion of biomasses due to their high Lewis acidity, which allows coordination to functionalized molecules and promotes cleavage and rearrangement reactions. For applications in biorefining zeolite porosity would ideally be optimized to avoid diffusional limitations, which otherwise may decrease reaction rates and restrict the conversion of bulky substrates. The synthesis of mesoporous zeolites can help alleviating limitations and is a central topic in heterogeneous catalysis, with many synthetic procedures for mesoporous zeolites proposed over the last decades. Here, we explore different syntheses routes to prepare Lewis acidic Sn-containing zeolites, and the main features of the prepared mesoporous materials are characterized. We investigate the correlation between different types of porosity and the activity for the conversion of sugars into methyl lactate. The monomer glucose, the dimer sucrose and the oligomer inulin are applied as model substrates for the reaction in order to probe the accessibility of molecules with different sizes to active sites in zeolites with different pore systems.

In this work, the dynamics of electronic energy transfer (EET) in bichromophoric donor-acceptor systems, obtained by functionalizing a calix[4]arene scaffold with two dyes, was experimentally and theoretically characterized. The investigated compounds are highly versatile, due to the possibility of linking the dye molecules to the cone or partial cone structure of the calix[4]arene, which directs the two active units to the same or opposite side of the scaffold, respectively. The dynamics and efficiency of the EET process between the donor and acceptor units was investigated and discussed through a combined experimental and theoretical approach, involving ultrafast pump-probe spectroscopy and density functional theory based characterization of the energetic and spectroscopic properties of the system. Our results suggest that the external medium strongly determines the particular conformation adopted by the bichromophores, with a direct effect on the extent of excitonic coupling between the dyes and hence on the dynamics of the EET process itself.

Two adult sport horses were referred for respiratory noise, exercise intolerance, coughing and nasal discharge containing food material. An asymmetrical, mild to moderate defect of the soft palate was diagnosed and surgically treated by laryngeal tie-forward procedure (LTFP). The immediate postoperative endoscopic images were satisfactory; the epiglottis was in normal alignment, dorsal to the soft palate defect in both cases. When contacted respectively four months and three years after surgery, owners of both horses declared being satisfied with the outcome of surgery as initial clinical signs had reduced or disappeared. Follow-up endoscopy showed some retraction of the larynx in both horses but the reduction of the palatal defect was adequate. This report demonstrates that horses can reach adulthood with certain soft palate defects and that severity of clinical signs varies between individuals. The authors suggest considering LTFP as an option in adult horses with small-to-moderate cleft palates causing clinical signs.

Chain-stoppers give rise to ring-chain equilibria in dynamic libraries allowing determination of thermodynamic effective molarities for macrocylisation reactions.

Titrations and functional assays are combined to gain insight into the stoichiometric correlations for alkali ions and the tin active sites in Sn-Beta, and show that three different states with distinct catalytic properties exist.