This report describes the in vitro LTB anastomosis of a large diameter cylindrical organ. The immediate bond strength, as estimated by burst pressure measurements, was double compared to sutured anastomosis.
Laser tissue soldering is a technique for the closure of incisions, which provides an immediate air-and watertight bond. Previous studies have shown that this method can bond tracheal incisions with the aid of stay sutures or solid albumin strips. In this study we investigated whether soldering using a diode laser and indocyanine green (ICG) dyed liquid albumin solution as solder, was efficacious for the repair of tracheal incisions without the need for additional strengthening aids. A transverse incision was made in isolated pig tracheas. The incision was smeared with liquid solder composed of 42% bovine albumin and 0.1 mg/ml ICG, and soldered using a temperature-controlled fiberoptic diode laser system. The soldered tracheal ends were sealed and the burst pressure measured. In a series of experiments the burst pressure was found to be higher than 382 mm Hg. These preliminary results demonstrate that diode laser tissue soldering of tracheal incisions provides better results than with a CO2 laser, and that it is possible to achieve considerable soldering strength without the use of any additional strengthening procedures.
Resection of a segment of the trachea is a procedure applied for the removal of cervical tumors invading the trachea, or for the treatment of severe tracheal stenosis. The current method of anastomosis is based on multiple sutures. The main drawbacks of this method are: 1) A long procedure time, 2) An air leakage, and 3) An inflammatory response to the sutures.In this study we evaluated the feasibility and effectiveness of the use of temperature controlled CO 2 laser soldering of incisions in pig tracheas in vitro. A transverse incision was made in a separated pig trachea. A flexible albumin band was prepared and was laser soldered with albumin solder to the outer surface of the trachea, covering the incision. The soldered trachea ends were sealed and the burst pressure was measured. In a series of in vitro experiments, the mean burst pressure was found to be 230 mm Hg. These preliminary results demonstrated that laser soldering using a flexible albumin band may be a useful method for sealing an incision in the trachea.
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