The effect of growing conditions on starch and protein content in triticale grain and amylose content in starch was studied on winter triticale cvs. Kitaro, lupus, lamberto and ticino harvested in 2006 and 2007 in Humpolec and Pernolec, Czech republic. Dry matter of the investigated triticale grain contained 62.4-70.9% of starch. The starch content and amylose content in starch were significantly affected by cultivar and year. Statistical analysis did not confirm that starch content and amylose content in starch were significantly influenced by growing variant or location. The protein content was significantly affected only by year. The differences in protein content among cultivars, growing variants and locations were not significant. The protein content was negatively correlated with starch content (r = -0.83). Statistical analysis of data also confirmed a positive correlation between the starch content and amylose content in starch (r = 0.45).
The effect of F. culmorum inoculation on the yield and quality of grain of AF Cesar and AF Lucius barley varieties was monitored. Field experiments were conducted between 2015-2017 at two locations. In grain harvested from plots grown under natural infection conditions, the deoxynivalenol content was very low. Inoculation caused a decline in germination, a certain reduction in yield and a change in some qualitative parameters (reduction in protein and fiber content, increase in starch content), but the effect of inoculation was weak and significantly influenced by the environment and the variety. The β-glucan content was not affected by inoculation. The varieties differed significantly from each other in β-glucan content (AF Cesar > AF Lucius). The deoxynivalenol content of both varieties was comparable after the inoculation while it differed under conditions of the natural infection (AF Cesar < AF Lucius).
Deoxynivalenol and zearalenone content and the presence of Fusarium species in organically and conventionally grown wheat harvested in the Czech Republic during 2015-2017 were studied. Mycotoxin contamination was compared using two approaches. The first was based on samples taken from randomly selected farms in individual regions of the Czech Republic, both organic (154 samples) and conventional (330 samples). In the second approach, a sample set of conventional wheat compatible with organic one was formed, with samples paired according to the preceding crop and region of harvest. Using the first approach, mycotoxins were shown to be higher in conventional wheat; however, there was no difference in mycotoxin contamination between organic and conventional wheat using the second approach. Eight wheat samples with mycotoxin content above the EU limits were found, seven for deoxynivalenol (2.1%) and one for zearalenone (0.3%), all of them originated from conventional farming system. Six of them had maize as the preceding crop. The presence of five Fusarium species (Fusarium avenaceum, Fusarium culmorum, Fusarium graminearum, Fusarium poae and Fusarium sporotrichioides) was compared for the compatible wheat sample sets (second approach). The predominant species were found to be F. poae and F. graminearum in both the organic and conventional wheat. Harvest year significantly influenced both the occurrence of the main Fusarium species and the level of mycotoxin contamination. The study confirmed that (a) organic farming system was able to keep mycotoxin contamination of wheat at a low level, (b) in studies on the significance of organic/conventional agricultural practice on mycotoxin levels in cereals, it is important to pay attention to common production and environmental variables.
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