Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials. Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups (n = 10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn's test were used for multiple comparisons. Mann Whitney U test was used to compare the surface roughness. Results. Statistically significant difference (P < 0.05) was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins. Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.
The aim of this study was to compare the efficacy of using a dishwasher or different chemical agents, including 0.12% chlorhexidine gluconate, 2% sodium hypochlorite (NaOCl), a mouthrinse containing essential oils and alcohol, and 50% white vinegar, for toothbrush disinfection. Sixty volunteers were divided into five experimental groups and one control group (n = 10). Participants brushed their teeth using toothbrushes with standard bristles, and they disinfected the toothbrushes according to instructed methods. Bacterial contamination of the toothbrushes was compared between the experimental groups and the control group. Data were analyzed by Kruskal-Wallis and Duncan's multiple range tests, with 95% confidence intervals for multiple comparisons. Bacterial contamination of toothbrushes from individuals in the experimental groups differed from those in the control group (p < 0.05). The most effective method for elimination of all tested bacterial species was 50% white vinegar, followed in order by 2% NaOCl, mouthrinse containing essential oils and alcohol, 0.12% chlorhexidine gluconate, dishwasher use, and tap water (control). The results of this study show that the most effective method for disinfecting toothbrushes was submersion in 50% white vinegar, which is cost-effective, easy to access, and appropriate for household use.
Objective. This study aimed to evaluate the effectiveness of alternative methods for toothbrush disinfection. Methods. Two-hundred eighty toothbrushes were included in the study. The toothbrushes were divided into 7 groups and were contaminated by standardized suspensions of Lactobacillus rhamnosus (L. rhamnosus), Streptococcus mutans (S. mutans), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). The following disinfectants were tested: 1% sodium hypochlorite (NaOCl), 100% and 50% white vinegar, microwave (MW) oven, ultraviolet (UV) sanitizer, and mouth rinse-containing propolis (MCP). Data were analyzed with Kruskal Wallis and Dunn's tests. Results. Statistically significant differences were found between different methods and control group for all tested bacteria. There were statistically significant differences between all test groups for all microorganisms. MW was the most effective for L. rhamnosus and 100% white vinegar was the most effective method for S. mutans and S. aureus. NaOCl was the most effective for E. coli. Conclusion. This study showed that 100% white vinegar was considered to be effective for tested microorganisms. Similarly, 1% NaOCl is cost-effective, easily accessible, and comparatively effective for toothbrush disinfection. Because these agents are nontoxic, cost-effective and easily accessible, they may be appropriate for household use.
The awareness of some harmful side effects of the chemicals contained in synthetic cosmetics has increased the demand for herbal-based cosmetic products today. White pitahaya fruit and peel methanol extracts were prepared to determine their usage potential in the cosmetic industry. Firstly, we investigated their antimicrobial activity against some test microorganisms using the disc diffusion assay. We also determined their minimal inhibition and minimal bactericidal or fungicidal concentrations. Then, we assayed the antimicrobial activity of a commercial cream containing white pitahaya extracts and the probiotic Lactobacillus fermentum MA-7 strain against the test microorganisms. Finally, we measured the sun protection factors of the white pitahaya fruit and peel extracts and the cream with the extracts. The white pitahaya fruit and peel extracts exhibited antimicrobial activity against the test microorganisms. The cream formulation containing a pitahaya fruit extract had the highest inhibition zone diameter of 11.25 mm against Escherichia coli O157:H7. The highest sun protection value among the extracts and cream with extracts was determined for peel extract as 6.66 and 23.34, respectively. The results indicate that pitahaya fruit and peel extracts have effective antibacterial and antifungal properties, as well as high sun protection factors, and therefore they could be used as natural preservatives in the cosmetic industry.
Bu çalışmanın amacı, annelerin, çocukları için reçete edilmiş ağızdan verilen toz antibiyotikleri hazırlama ve reçete edilen dozda ölçme yeterliliklerini değerlendirmektir.Gereç ve Yöntem: Bu tanımlayıcı araştırma, eczanelere, çocukları için reçete edilmiş ağızdan kullanılan toz antibiyotikleri almak üzere başvuran 99 anne ile gerçekleştirilmiştir. Anneler toz antibiyotiği hazırlarken gözlenmiş ve hazırlanan süspansiyon 10 ml'lik cam pipet ile ölçülmüştür. Ölçümün hekimin reçete ettiği doza göre ± 1 ml aralığında olması doğru ölçüm olarak kabul edilmiştir.Bulgular: Annelerin %27,3'ü ilacı ölçülmesi gerekenden daha düşük dozlarda hazırlarken %2'si de daha fazla dozda hazırlamıştır (p<0.001). Annelerin eğitim düzeyinin yüksek olması ve ölçüm için şırınga kullanmaları ile doğru ölçüm arasında istatistiksel olarak anlamlı bir ilişki bulunmuştur.Sonuç: Bu çalışmada annelerin ağızdan verilen toz antibiyotikleri hazırlamada yeterli olmadıkları ve yanlış dozlarda ölçeklendirdikleri saptanmıştır. Bulgularımız, doğru ölçüm yapılabilmesi için doktorların anneleri şırınga kullanmaya teşvik etmesi gerektiğini düşündürmektedir.
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